Detection of Mycobacterium leprae In Formalin-Fixed Paraffin-Embedded Sample By Fite-Faraco Staining and Polymerase Chain Reaction

被引：3

作者：

Sandhika, Willy ^{[1
]}

Adriaty, Dinar ^{[2
]}

Agusni, Indropo ^{[3
]}

机构：

[1] Airlangga Univ, Fac Med, Dept Anat Pathol, Surabaya, Indonesia

[2] Airlangga Univ, Inst Trop Dis, Surabaya, Indonesia

[3] Airlangga Univ, Fac Med, Dept Dermatovenereol, Surabaya, Indonesia

来源：

MOLECULAR AND CELLULAR LIFE SCIENCES: INFECTIOUS DISEASES, BIOCHEMISTRY AND STRUCTURAL BIOLOGY 2015 CONFERENCE | 2016年 / 18卷

关键词：

PCR mycobacteria; fite-faraco; leprosy skin biopsy; LEPROSY PATIENTS; DNA;

D O I：

10.1016/j.proche.2016.01.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Leprosy is a chronic infectious disease caused by Mycobacterium leprae. The identification of mycobacteria in tissue sections can be made through a microscopic examination with fite-faraco staining or PCR method. Paraffin blocks from four patients with leprosy were retrieved from The Pathologic Department of Dr. Soetomo Hospital, Surabaya. Two cases were from paucibacillary leprosy patients with no mycobacteria stained by fite-faraco. PCR assay showed a negative result. The other two cases were multibacillary leprosy with many bacteria stained by fite-faraco. PCR assay showed a positive result. (C) 2016 The Authors. Published by Elsevier B.V.

引用

页码：231 / 236

页数：6

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