Mesoflavibacter sabulilitoris sp nov., isolated from seashore sand

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, gliding and rod-shaped bacterial strain, designated GJMS-9(T), was isolated from seashore sand collected at Geoje island in the South Sea, South Korea. Strain GJMS-9(T) grew optimally at 30 degrees C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GJMS-9(T) clustered with the type strain of Mesoflavibacter zeaxanthinifaciens, showing the highest sequence similarity of 99.1%. Strain GJMS-9(T) exhibited 16S rRNA gene sequence similarity of 96.5% to the type strain of Mesoflavibacter aestuarii and of less than 96.1% to the type strains of other recognized species. Strain GJMS-9(T) contained MK-6 as the only menaquinone and iso-C-15:1 G, iso-C-16:0 3-OH, iso-C-15:0 and iso-C-17:0 3-OH as the major fatty acids. The polar lipid profile of strain GJMS-9(T) containing phosphatidylethanolamine, one unidentified lipid and one unidentified glycolipid as major components was similar to that of the type strain of M. zeaxanthinifaciens. The DNA G + C content of strain GJMS-9(T) was 32.2 mol% and its DNA-DNA relatedness with M. zeaxanthinifaciens DSM 18436(T) was 38 +/- 6.1 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GJMS-9(T) is separated from other species of the genus Mesoflavibacter. On the basis of the data presented, strain GJMS-9(T) is considered to represent a novel species of the genus Mesoflavibacter, for which the name Mesoflavibacter sabulilitoris sp. nov. is proposed. The type strain is GJMS-9(T) (=KCTC 42117(T)=CECT 8597(T)).