A Brief Review of Molecular Techniques to Assess Plant Diversity

被引:88
|
作者
Arif, Ibrahim A. [1 ]
Bakir, Mohammad A. [1 ]
Khan, Haseeb A. [1 ]
Al Farhan, Ahmad H. [1 ]
Al Homaidan, Ali A. [1 ]
Bahkali, Ali H. [1 ]
Al Sadoon, Mohammad [1 ]
Shobrak, Mohammad [1 ]
机构
[1] King Saud Univ, Coll Sci, Prince Sultan Res Chair Environm & Wildlife, Mol Fingerprinting & Biodivers Unit, Riyadh, Saudi Arabia
关键词
molecular diversity; DNA fingerprinting; plant species; conservation genetics; GENETIC DIVERSITY; MICROSATELLITE LOCI; ENDANGERED PLANT; CONSERVATION GENETICS; SEQUENCE INFORMATION; GERMPLASM COLLECTION; NUCLEOTIDE-SEQUENCES; AFLP MARKERS; DNA BARCODES; GENERATION;
D O I
10.3390/ijms11052079
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Massive loss of valuable plant species in the past centuries and its adverse impact on environmental and socioeconomic values has triggered the conservation of plant resources. Appropriate identification and characterization of plant materials is essential for the successful conservation of plant resources and to ensure their sustainable use. Molecular tools developed in the past few years provide easy, less laborious means for assigning known and unknown plant taxa. These techniques answer many new evolutionary and taxonomic questions, which were not previously possible with only phenotypic methods. Molecular techniques such as DNA barcoding, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), microsatellites and single nucleotide polymorphisms (SNP) have recently been used for plant diversity studies. Each technique has its own advantages and limitations. These techniques differ in their resolving power to detect genetic differences, type of data they generate and their applicability to particular taxonomic levels. This review presents a basic description of different molecular techniques that can be utilized for DNA fingerprinting and molecular diversity analysis of plant species.
引用
收藏
页码:2079 / 2096
页数:18
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