IL-2 Inhibited the Generation of CD4+ Memory T Cells

被引：3
|
作者：
Lu, Hongjuan [1 ,2 ,3 ]
Chen, Jie [4 ]
Nie, Xuefeng [5 ]
Liu, Cong [6 ]
Sun, Weimin [1 ,2 ]
机构：
[1] Second Mil Med Univ, Natl Key Lab Med Immunol, Dept Immunol, Shanghai 200433, Peoples R China
[2] Second Mil Med Univ, Inst Immunol, Shanghai 200433, Peoples R China
[3] Second Mil Med Univ, Changzheng Hosp, Rheumatism Immun Dept, Shanghai 200433, Peoples R China
[4] PLA, Hosp 117, Dept Ophthalmol, Hangzhou 310013, Zhejiang, Peoples R China
[5] Chengdu Mil Reg, Dept Ctr Dis Prevent & Control, Chengdu 610021, Peoples R China
[6] Second Mil Med Univ, Fac Naval Med, Dept Radiat Med, Shanghai 200433, Peoples R China
来源：
CELL BIOCHEMISTRY AND BIOPHYSICS | 2014年 / 70卷 / 03期
基金：
中国国家自然科学基金;
关键词：
IL-2; CD4(+) memory T cell; IL-15; PI3K/AKT; REGULATORY DENDRITIC CELLS; IMMUNOLOGICAL MEMORY; GAMMA-CHAIN; B-CELL; PROLIFERATION; INTERLEUKIN-7; HOMEOSTASIS; CYTOKINES; PROMOTES; SURVIVAL;
D O I：
10.1007/s12013-014-0117-z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The survival of T cells at different stages of development is dependent on extrinsic signals. IL-7 is necessary for the development of memory T cells. IL-7 could induce and maintain the differentiation, survival, and proliferation of CD4(+) memory T cells, and the roles of IL-2 and IL-15 in the generation of CD4(+) memory T cells were still unclear. A CD4(+) memory T cells in vitro generated system by adding IL-7. The phenotype of CD4(+) memory T cells was identified by FACS. The cells proliferation was analyzed by CFSE staining. The involved signal pathways were analyzed by Western blot. We found that IL-2, not IL-15, could inhibit CD4(+) memory T cells generation. Western blot showed that IL-7 up-regulated the P-STAT5A expression and down-regulated Bax expression, IL-2 reduced the effect of IL-7. Besides, IL-2-combined IL-7 up-regulated the P-AKT and Foxo3a expression a little. In conclusion, our data revealed the inhibitory role of IL-2 in CD4(+) memory T cells generation and indicated that PI3K/AKT signal pathway was involved.
引用
收藏
页码：1705 / 1711
页数：7
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