Effects of wheat intercropping on the nitrogen metabolism during senescence of cucumber leaves

被引:6
|
作者
Li, Y. Y. [1 ,2 ]
Li, P. Y. [1 ]
Wu, F. Z. [1 ]
Zhou, X. G. [1 ]
Han, Q. S. [1 ]
Zhang, J. [1 ]
Liu, S. Y. [1 ]
Liu, S. W. [1 ,2 ]
机构
[1] Northeast Agr Univ, Coll Hort & Landscape, Mucai 59, Harbin 150030, Heilongjiang, Peoples R China
[2] Northeast Agr Univ, Minist Agr, Key Lab Biol & Germplasm Enhancement Hort Crops N, Mucai 59, Harbin 150030, Heilongjiang, Peoples R China
来源
ALLELOPATHY JOURNAL | 2018年 / 45卷 / 01期
关键词
Cucumber; Cucumis sativus L; enzymes; intercropping; leaf senescence; nitrogen metabolism; qRT-PCR; Triticum aestivum L; wheat; PHYSIOLOGICAL-CHARACTERISTICS; GLUTAMINE-SYNTHETASE; SEEDLINGS; GROWTH; NITRATE; MAIZE; ANTIOXIDANT; EXPRESSION; INDICATORS; MELATONIN;
D O I
10.26651/allelo.j./2018-45-1-1172
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
In Greenhouse experiment, we studied the effects of wheat (Triticum aestivum L.) intercropping on the nitrogen metabolism during the senescence of cucumber (Cucumis sativus L.) leaves. The results showed that compared with cucumber monoculture, intercropping with wheat increased the content of soluble protein, nitrate nitrogen, free amino acid and proline and enhanced the activity of nitrate reductase (NR), glutamine synthase (GS), glutamate dehydrogenase (GDH) and glutamate synthase (GOGAT) of cucumber leaves. The qRT-PCR analysis revealed that compared with cucumber monoculture, the expression of N1, N2 and N3, which belong to NRT (Nitrate Transporter Protein) family and Al, which belong to AMT (Ammonium Transporter Protein) family and GS1 (cytosolic glutamine synthase gene) were up-regulated in intercropped cucumber. In short, the cultivation mode of intercropping with wheat increased the content of nitrogen compounds and enzyme activity and promoted the expression of genes related to nitrogen metabolism in cucumber leaves, which delayed the senescence of cucumber leaves.
引用
收藏
页码:13 / 28
页数:16
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