Immobilization of DNA on self-assembled monolayer

被引：2
|
作者：
Nakamura, F
Mitsui, K
Murase, T
Kobayashi, K
Hara, M
Knoll, W
Sasabe, H
机构：
[1] RIKEN, Biopolymer Phys Lab, Wako, Saitama 3510198, Japan
[2] RIKEN, Frontier Res Program, Wako, Saitama 3510198, Japan
来源：
MOLECULAR CRYSTALS AND LIQUID CRYSTALS | 2000年 / 349卷
关键词：
DNA; intercalation; self-assembled monolayer; surface plasmon resonance; IR-RA spectroscopy; atomic force microscope;
D O I：
10.1080/10587250008024904
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We attempted the immobilization of DNA onto self-assembled monolayer (SAM) which contains the intercalator for DNA. Anthryl moieties were attached to the terminal OH groups in SAM of 1-Mercapt-11-undecanol (MU-SAM) by esterification. In the case of the SAM containing anthryl moieties (Anth-SAM), surface plasmon resonance measurement indicates that the adsorption of DNA onto the SAM was observed in an aqueous DNA solution, and the DNA remained on the surface even after rinsing. Although the adsorption of DNA onto MU-SAM was also observed, little DNA remained on the SAM after rinsing. These results indicate that the anthracene which was attached to the SAM can interact with DNA because the binding ability was increased by introduction of anthracene moieties to the SAM.
引用
收藏
页码：219 / 222
页数：4
相关论文
共 50 条
  • [31] Subsecond Self-Assembled Monolayer Formation
    Korolkov, Vladimir V.
    Allen, Stephanie
    Roberts, Clive J.
    Tendler, Saul J. B.
    JOURNAL OF PHYSICAL CHEMISTRY C, 2010, 114 (45): : 19373 - 19377
  • [32] MORPHOLOGY OF A SELF-ASSEMBLED MONOLAYER OF A POLYMER
    STEINER, UB
    REHAHN, M
    CASERI, WR
    SUTER, UW
    MACROMOLECULES, 1994, 27 (07) : 1983 - 1984
  • [33] Calligraphy on self-assembled monolayer of supramolecules
    Wu, DM
    Yin, XB
    Zhang, X
    Tseng, HR
    Stoddart, JF
    2003 THIRD IEEE CONFERENCE ON NANOTECHNOLOGY, VOLS ONE AND TWO, PROCEEDINGS, 2003, : 606 - 608
  • [34] BINARY SELF-ASSEMBLED MONOLAYER FORMATION
    FRANK, CW
    MATHAUER, K
    YANG, J
    KATAOKA, D
    CHANG, Y
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1994, 208 : 22 - COLL
  • [35] Electrochemistry of a carotenoid self-assembled monolayer
    Ion, A
    Partali, V
    Sliwka, HR
    Banica, FG
    ELECTROCHEMISTRY COMMUNICATIONS, 2002, 4 (09) : 674 - 678
  • [36] Immobilization of single-stranded deoxyribonucleic acid on gold electrode with self-assembled aminoethanethiol monolayer for DNA electrochemical sensor applications
    Sun, XY
    He, PG
    Liu, SH
    Ye, JN
    Fang, YZ
    TALANTA, 1998, 47 (02) : 487 - 495
  • [37] Immobilization of Bovine Serum Albumin on self-assembled monolayer modified dielectric film for biosensor application
    Tan, OK
    Fang, XY
    Wei, Q
    Yao, MW
    Tjin, SC
    2005 IEEE SENSORS, VOLS 1 AND 2, 2005, : 397 - 400
  • [38] Area-selective immobilization of multi enzymes by using the reductive desorption of self-assembled monolayer
    Oyamatsu, D
    Kanaya, N
    Hirano, Y
    Nishizawa, M
    Matsue, T
    ELECTROCHEMISTRY, 2003, 71 (06) : 439 - 441
  • [39] Oriented immobilization of antibodies by a self-assembled monolayer of 2-(biotinamido)ethanethiol for immunoarray preparation
    Jung, SH
    Son, HY
    Yuk, JS
    Jung, JW
    Kim, KH
    Lee, CH
    Hwang, H
    Ha, KS
    COLLOIDS AND SURFACES B-BIOINTERFACES, 2006, 47 (01) : 107 - 111
  • [40] Immobilization of horseradish peroxidase on a self-assembled monolayer modified gold electrode for the detection of hydrogen peroxide
    Sun, JJ
    Fang, HQ
    Chen, HY
    ANALYST, 1998, 123 (06) : 1365 - 1368
12345