BSA Adsorption on Porous Scaffolds Prepared from BioPEGylated Poly(3-Hydroxybutyrate)

被引：4

作者：

Bonartsev, A. P. ^{[1
,2
]}

Voinova, V. V. ^{[1
,2
]}

Kuznetsova, E. S. ^{[2
]}

Zharkova, I. I. ^{[2
]}

Makhina, T. K. ^{[1
]}

Myshkina, V. L. ^{[1
]}

Chesnokova, D. V. ^{[2
]}

Kudryashova, K. S. ^{[2
]}

Feofanov, A. V. ^{[2
]}

Shaitan, K. V. ^{[2
]}

Bonartseva, G. A. ^{[1
]}

机构：

[1] Russian Acad Sci, Bach Inst Biochem, Fed Res Ctr Fundamentals Biotechnol, Moscow 119071, Russia

[2] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119234, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2018年 / 54卷 / 04期

基金：

俄罗斯科学基金会;

关键词：

poly(3-hydroxybutyrate); bovine serum albumin; bioPEGylation; polyethylene glycol; adsorption; AZOTOBACTER-CHROOCOCCUM; 7B; IN-VITRO; BIOCOMPATIBILITY; GROWTH; MICROSPHERES; CARRIERS; RELEASE; ALBUMIN; SYSTEMS; FILMS;

D O I：

10.1134/S0003683818040038

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Porous scaffolds for tissue engineering have been prepared from poly(3-hydroxybutyrate) (PHB) and a copolymer of poly(3-hydroxybutyrate) and polyethylene glycol (PHB-PEG) produced by bioPEGylation. The morphology of the scaffolds and their capacity for adsorption of the model protein bovine serum albumin (BSA) have been studied. Scaffolds produced from bioPEGylated PHB adsorbed more BSA, whereas the share of protein irreversibly adsorbed on these scaffolds was significantly lower (33%) than in the case of PHB homopolymer-based scaffolds (47%). The effect of protein adsorption on scaffold biocompatibility in vitro was tested in an experiment that involved the cultivation of fibroblasts (line COS-1) on the scaffolds. PHB-PEG scaffolds had a higher capacity for supporting cell growth than PHB-based scaffolds. Thus, the bioPEGylated PHB-based polymer scaffolds developed in the present study have considerable potential for use in soft tissue engineering.

引用

页码：379 / 386

页数：8

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