Polytetrafluoroethylene tape as a low-cost hydrophobic substrate for drop-coating deposition Raman spectroscopy of proteins

被引:7
|
作者
Smith, James [1 ]
Arnolds, Heike [1 ]
机构
[1] Univ Liverpool, Dept Chem, Crown St, Liverpool L69 7ZD, Merseyside, England
基金
英国工程与自然科学研究理事会;
关键词
DCDR; drop coating deposition Raman; hen egg-white lysozyme; human insulin; hydrophobic surface; IDENTIFICATION; FLUORESCENCE; SCATTERING;
D O I
10.1002/jrs.5371
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
Highly hydrophobic substrates are desirable for drop coating deposition Raman spectroscopy of proteins, because they concentrate the analyte during drying. Most of the commercial substrates used to date are comparatively expensive, whereas thiol or silane functionalisation of surfaces requires a degree of experience not available in a typical biology laboratory. Here, we show that polytetrafluoroethylene tape, commercially available as thread sealing tape, can be stretched across a washer to yield a highly hydrophobic surface, which is thin enough to produce only a very small Raman signal itself. We demonstrate that this simple substrate can be used to yield high-quality spectra of biomolecules such as human insulin and lysozyme in both native and fibrillated forms.
引用
收藏
页码:1236 / 1239
页数:4
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