PURIFICATION OF EXTRACELLULAR α-AMYLASE AND PROTEINASE FROM Bacillus subtilis SKB 256

被引：1
|
作者：
Radzhabov, U. R. [1 ]
Davranov, K. [1 ]
机构：
[1] Mirzo Ulugbek Natl Univ Uzbekistan, Tashkent, Uzbekistan
来源：
CHEMISTRY OF NATURAL COMPOUNDS | 2010年 / 46卷 / 03期
关键词：
Bacillus subtilis SKB 256; alpha-amylase; proteinase; biospecific chromatography;
D O I：
10.1007/s10600-010-9635-0
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
The enzymes alpha-amylase and proteinase with specific activity 310 and 68-72 units/mg, respectively, were isolated from Bacillus subtilis SKB 256 culture medium. The enzyme preparations were purified and alpha-amylase and proteinase were separated using biospecific chromatography. Gel filtration of alpha-amylase was performed over a column (20 x 600 mm) of TSK HW-55F gel (Toyopearl, Japan).
引用
收藏
页码：436 / 439
页数：4
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