Mass spectrometric detection of the amino acid sequence polymorphism of the hepatitis C virus antigen

被引：5
|
作者：
Kaysheva, A. L. [1 ]
Ivanov, Yu. D. [1 ]
Frantsuzov, P. A. [1 ]
Krohin, N. V. [1 ]
Pavlova, T. I. [1 ]
Uchaikin, V. F. [1 ]
Konev, V. A. [1 ]
Kovalev, O. B. [1 ]
Ziborov, V. S. [1 ]
Archakov, A. I. [1 ]
机构：
[1] Inst Biomed Chem, Pogodinskaya St 10, Moscow 119121, Russia
来源：
JOURNAL OF VIROLOGICAL METHODS | 2016年 / 229卷
关键词：
Hepatitis C; HCVcoreAg detection; Mass spectrometry; SAP; CORE PROTEIN; GENOTYPES;
D O I：
10.1016/j.jviromet.2015.12.012
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for detection and identification of the hepatitis C virus antigen (HCVcoreAg) in human serum with consideration for possible amino acid substitutions is proposed. The method is based on a combination of biospecific capturing and concentrating of the target protein on the surface of the chip for atomic force microscope (AFM chip) with subsequent protein identification by tandem mass spectrometric (MS/MS) analysis. Biospecific AFM-capturing of viral particles containing HCVcoreAg from serum samples was performed by use of AFM chips with monoclonal antibodies (anti-HCVcore) covalently immobilized on the surface. Biospecific complexes were registered and counted by AFM. Further MS/MS analysis allowed to reliably identify the HCVcoreAg in the complexes formed on the AFM chip surface. Analysis of MS/MS spectra, with the account taken of the possible polymorphisms in the amino acid sequence of the HCVcoreAg, enabled us to increase the number of identified peptides. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：86 / 90
页数：5
相关论文
共 50 条
  • [21] Detection of hepatitis B virus YMDD variants using mass spectrometric analysis of oligonucleotide fragments
    Hong, SP
    Kim, NK
    Hwang, SG
    Chung, HJ
    Kim, S
    Han, JH
    Kim, HT
    Rim, KS
    Kang, MS
    Yoo, W
    Kim, SO
    JOURNAL OF HEPATOLOGY, 2004, 40 (05) : 837 - 844
  • [22] Mass spectrometric amino acid structure determination in ergopeptines
    Halada, P
    Sedmera, P
    Havlícek, V
    Jegorov, A
    Cvak, L
    Ryska, M
    EUROPEAN MASS SPECTROMETRY, 1998, 4 (05): : 385 - 392
  • [23] Mass spectrometric study of amino acid–triethylborane chelates
    Waldemar Iwanek
    Alicja Wzorek
    Zdzisława Nowakowska
    Grzegorz Schroeder
    Monatshefte für Chemie - Chemical Monthly, 2009, 140 : 359 - 364
  • [24] MASS SPECTROMETRIC STUDIES ON AMINO ACID AND PEPTIDE DERIVATIVES
    ANDERSSON, CO
    ACTA CHEMICA SCANDINAVICA, 1958, 12 (06): : 1353 - 1353
  • [25] Conservation of hepatitis C virus nonstructural protein 3 amino acid sequence in viral isolates during liver transplantation
    Mello, I. M. V. G. C.
    Thumann, C.
    Schvoerer, E.
    Soulier, E.
    Pinho, J. R. R.
    Silvestre, D. A. M. M.
    Queiroz, A. T. L.
    Wolf, P.
    Baumert, T. F.
    Keller, F. S.
    Pereira, C. A.
    JOURNAL OF VIRAL HEPATITIS, 2009, 16 (10) : 732 - 737
  • [26] Hepatitis C virus F protein sequence reveals a lack of functional constraints and a variable pattern of amino acid substitution
    Cristina, J
    Lopez, F
    Moratorio, G
    López, L
    Vasquez, S
    García-Aguirre, L
    Chunga, A
    JOURNAL OF GENERAL VIROLOGY, 2005, 86 : 115 - 120
  • [27] Combined detection of hepatitis C virus core antigen and antibody as an alternative to nucleic acid testing in blood screening
    不详
    VOX SANGUINIS, 2005, 89 : 21 - 21
  • [28] Is an assay for simultaneous detection of hepatitis C virus core antigen and antibody a valuable alternative to nucleic acid testing?
    Laperche, S
    Elghouzzi, MH
    Morel, P
    Asso-Bonnet, M
    Le Marrec, N
    Girault, A
    Servant-Delmas, A
    Bouchardeau, F
    Deschaseaux, M
    Piquet, Y
    TRANSFUSION, 2005, 45 (12) : 1965 - 1972
  • [29] Novel hepatitis C virus genotyping method based on mass spectrometric analysis of oligonucleotide fragments
    Kim, YJ
    Chung, HJ
    Ji, MS
    Kim, KM
    Yoon, JH
    Kim, BG
    Lee, HS
    Kim, CY
    Kim, SO
    Yoo, W
    Hong, SP
    GASTROENTEROLOGY, 2004, 126 (04) : A741 - A741
  • [30] Miscibility of hepatitis A synthetic antigen peptides with lipid monolayers: Effect of the amino acid sequence change
    Sospedra, P
    Espina, M
    Alsina, MA
    Haro, MAI
    Mestres, C
    JOURNAL OF PHYSICAL CHEMISTRY B, 2003, 107 (01): : 203 - 208
12345