Mass spectrometric detection of the amino acid sequence polymorphism of the hepatitis C virus antigen

被引：5
|
作者：
Kaysheva, A. L. [1 ]
Ivanov, Yu. D. [1 ]
Frantsuzov, P. A. [1 ]
Krohin, N. V. [1 ]
Pavlova, T. I. [1 ]
Uchaikin, V. F. [1 ]
Konev, V. A. [1 ]
Kovalev, O. B. [1 ]
Ziborov, V. S. [1 ]
Archakov, A. I. [1 ]
机构：
[1] Inst Biomed Chem, Pogodinskaya St 10, Moscow 119121, Russia
来源：
JOURNAL OF VIROLOGICAL METHODS | 2016年 / 229卷
关键词：
Hepatitis C; HCVcoreAg detection; Mass spectrometry; SAP; CORE PROTEIN; GENOTYPES;
D O I：
10.1016/j.jviromet.2015.12.012
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for detection and identification of the hepatitis C virus antigen (HCVcoreAg) in human serum with consideration for possible amino acid substitutions is proposed. The method is based on a combination of biospecific capturing and concentrating of the target protein on the surface of the chip for atomic force microscope (AFM chip) with subsequent protein identification by tandem mass spectrometric (MS/MS) analysis. Biospecific AFM-capturing of viral particles containing HCVcoreAg from serum samples was performed by use of AFM chips with monoclonal antibodies (anti-HCVcore) covalently immobilized on the surface. Biospecific complexes were registered and counted by AFM. Further MS/MS analysis allowed to reliably identify the HCVcoreAg in the complexes formed on the AFM chip surface. Analysis of MS/MS spectra, with the account taken of the possible polymorphisms in the amino acid sequence of the HCVcoreAg, enabled us to increase the number of identified peptides. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：86 / 90
页数：5
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