Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis

被引:33
|
作者
Khamrin, Pattara
Maneekarn, Niwat
Peerakome, Supatra
Tonusin, Supin
Phan, Tung Gia
Okitsu, Shoko
Ushijima, Hiroshi
机构
[1] Univ Tokyo, Grad Sch Med, Inst Int Hlth, Dept Dev Med Sci,Bunkyo Ku, Tokyo 1130033, Japan
[2] Chiang Mai Univ, Fac Med, Dept Microbiol, Chiang Mai 50000, Thailand
[3] McCormick Hosp, Dept Pediat, Chiang Mai, Thailand
关键词
rotavirus; G3P[9] genotype; AU-1-like; VP4; VP7;
D O I
10.1002/jmv.20840
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In 2004, an epidemiological survey of human rotavirus infection in Chiang Mai, Thailand detected two uncommon human rotavirus strains (CMH120/04 and CMH134/04) bearing AU-1-like G3P[9] genotypes in 1 year old children hospitalized with acute gastroenteritis. The CMH120/04 and CMH134/04 rotavirus strains were characterized by molecular analyses of their VP6, VP7, VP8*, and NSP4 gene segments as well as the determination of RNA patterns by polyacrylamide gel electrophoresis (PAGE). Analysis of the VP8* gene revealed a high level of amino acid sequence identities with those of P[9] rotavirus reference strains, ranging from 94.9% to 98.3%. The highest identities were shared with the human rotavirus AU-1 strain at 97.8% and 98.3% for CMH120/04 and CMH134/04 strains, respectively. Analysis of the VP7 gene sequence revealed the highest identities with G3 human rotavirus strain KC814 at 96.6% and 96.2% for CMH120/04 and CMH134/ 04 strains, respectively. Based on the analyses of VP7 and VP8* genes, CMH120/04 and CMH134/04 belonged to G3P[91 genotypes. In addition, analyses of VP6 and NSP4 sequences revealed a VP6 subgroup (SG) 1, with NSP4 genetic group C specificities. Moreover, both strains displayed a long RNA electrophoretic pattern. The finding of uncommon G3P[91 rotaviruses in pediatric patients provided additional evidence of the genetic/antigenic diversities of human group A rotaviruses in the Chiang Mai area of Thailand, J. Med. Virol. 79:843-851, 2007. (C) 2007 Wiley-Liss, Inc.
引用
收藏
页码:843 / 851
页数:9
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