Effect of media on spore yield and thermal resistance of Bacillus stearothermophilus

被引：0
|
作者：
Penna, TCV [1 ]
Machoshvili, IA [1 ]
Ishii, M [1 ]
机构：
[1] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, Sch Pharmaceut Sci, BR-04607000 Sao Paulo, SP, Brazil
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2003年 / 105卷
关键词：
Bacillus stearothermophilus; thermoresistance; sporulation; bioindicator;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The interference of eight components in the yield of sporulation and thermal resistance to moist heat (121degreesC) of Bacillus stearothermophilus spores suspended in 0.02 M calcium acetate solution and inoculated on paper strips previously treated with calcium acetate/calcium hydroxide was studied. The spore yield of 1.0 x 10(8)/mL was developed at 62degreesC in 17 media containing different concentrations Of D-glucose, sodium chloride, L-glutamic acid, yeast extract, peptone, manganese sulfate, potassium phosphate, and ammonium phosphate. The combined effects of yeast extract, peptone, and glucose contributed positively to the spore yield and to the stability of the thermal resistance of both spores in suspension and on strips.
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页码：287 / 294
页数：8
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