An optimized high-throughput fluorescence plate reader-based RSV neutralization assay

被引：1

作者：

Sun, Yong-Peng ^{[1
]}

Zhang, Wei ^{[2
]}

Zhao, Qin-Jian ^{[1
]}

Cao, Jian-Li ^{[2
]}

Zhang, Lu-Jing ^{[1
]}

Xiang, Jiang-Yan ^{[2
]}

Si, Jun-Yu ^{[2
]}

Lin, Xue ^{[1
]}

Chen, Li ^{[1
]}

Zheng, Zi-Zheng ^{[1
]}

Xia, Ning-Shao ^{[1
,2
]}

机构：

[1] Xiamen Univ, State Key Lab Mol Vaccinol & Mol Diagnost, Natl Inst Diagnost & Vaccine Dev Infect Dis, Sch Publ Hlth, Xiamen 361002, Fujian, Peoples R China

[2] Xiamen Univ, State Key Lab Mol Vaccinol & Mol Diagnost, Natl Inst Diagnost & Vaccine Dev Infect Dis, Sch Publ Hlth,Sch Life Sci, Xiamen 361002, Fujian, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2018年 / 260卷

基金：

中国国家自然科学基金;

关键词：

Fluorescence plate reader; Flow cytometry; RSV; Neutralization assay; RESPIRATORY SYNCYTIAL VIRUS; HEPATITIS-E VIRUS; INFECTION; ANTIBODY; RISK; EFFICIENT; VACCINES; INFANTS;

D O I：

10.1016/j.jviromet.2018.07.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A licensed vaccine for respiratory syncytial virus (RSV) has yet to be developed, and a reliable and repeatable neutralizing assay is indispensable for vaccine development. Here, we demonstrated an optimized high throughput RSV neutralization assay that utilizes a fluorescence plate reader (reader) as a substitute for flow cytometry to detect fluorescent signals in RSV-A2 mKate-infected cells. Furthermore, this study tested the influence of virus input and infectivity on the neutralizing assay and highlighted critical factors (together with a suggested protocol) for obtaining stable data using this assay.

引用

页码：34 / 40

页数：7

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