Immunolocalization of the Anti-Mullerian Hormone (AMH) in Caprine Follicles and the Effects of AMH on In Vitro Culture of Caprine Pre-antral Follicles Enclosed in Ovarian Tissue

Contents The aims of this study were to evaluate the localization, by immunohistochemistry, of the anti-Mullerian hormone (AMH) in goat ovaries and to investigate its effects on the invitro survival and development of caprine pre-antral follicles enclosed in fragments of ovarian tissue. Pre-antral follicles were cultured invitro for 1 or 7days in -MEM+ in the absence or presence of kit ligand (KL; 50ng/ml, positive control) or AMH (50 or 150ng/ml). The results showed that AMH was localized in oocytes and granulosa cells from the primordial follicle to antral follicle stages. Addition of AMH maintained the percentage of developing follicles, similar to that in the uncultured control; however, the percentage of developing follicles was significantly lower than that in the cultured control and KL. Nonetheless, addition of AMH to the culture medium did not affect survival rates and follicular growth. In conclusion, this study demonstrated that the expression of AMH varies according to the compartment and stage of follicular development. Furthermore, AMH inhibits the activation of caprine primordial follicles.