Discovery of chebulagic acid and punicalagin as novel allosteric inhibitors of SARS-CoV-2 3CLpro

被引：50
|
作者：
Du, Ruikun [1 ,2 ,3 ]
Cooper, Laura [4 ]
Chen, Zinuo [1 ]
Lee, Hyun [5 ]
Rong, Lijun [4 ]
Cui, Qinghua [1 ,2 ,3 ]
机构：
[1] Shandong Univ Tradit Chinese Med, Coll Pharm, Jinan 250355, Peoples R China
[2] Shandong Univ Tradit Chinese Med, Expt Ctr, Jinan 250355, Peoples R China
[3] Shandong Univ Tradit Chinese Med, Qingdao Acad Chinese Med Sci, Qingdao 266122, Peoples R China
[4] Univ Illinois, Coll Med, Dept Microbiol & Immunol, Chicago, IL 60612 USA
[5] Univ Illinois, Dept Pharmaceut Sci, Ctr Bimol Sci, Coll Pharm,Biophys Core Res Resources Ctr, Chicago, IL 60607 USA
来源：
ANTIVIRAL RESEARCH | 2021年 / 190卷
关键词：
SARS-CoV-2; Chebulagic acid; Punicalagin; 3CLpro; Allosteric inhibitor; COVALENT;
D O I：
10.1016/j.antiviral.2021.105075
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The emerging SARS-CoV-2 infection is the cause of the global COVID-19 pandemic. To date, there are limited therapeutic options available to fight this disease. Here we examined the inhibitory abilities of two broad-spectrum antiviral natural products chebulagic acid (CHLA) and punicalagin (PUG) against SARS-CoV-2 viral replication. Both CHLA and PUG reduced virus-induced plaque formation in Vero-E6 monolayer at noncytotoxic concentrations, by targeting the enzymatic activity of viral 3-chymotrypsin-like cysteine protease (3CL(pro)) as allosteric regulators. Our study demonstrates the potential use of CHLA and PUG as novel COVID-19 therapies.
引用
收藏
页数：7
相关论文
共 50 条
  • [31] Prioritisation of Compounds for 3CLpro Inhibitor Development on SARS-CoV-2 Variants
    Jukic, Marko
    Skrlj, Blaz
    Tomsic, Gasper
    Plesko, Sebastian
    Podlipnik, Crtomir
    Bren, Urban
    MOLECULES, 2021, 26 (10):
  • [32] Label-free duplex SAMDI-MS screen reveals novel SARS-CoV-2 3CLpro inhibitors
    Scholle, Michael D.
    O'Kane, Patrick T.
    Dib, Sandra
    Gurard-Levin, Zachary A.
    ANTIVIRAL RESEARCH, 2022, 200
  • [33] Design, synthesis and biological evaluation of peptidomimetic benzothiazolyl ketones as 3CLpro inhibitors against SARS-CoV-2
    Yang, Hanxi
    You, Mengyuan
    Shu, Xiaoyang
    Zhen, Jingyao
    Zhu, Mengwei
    Fu, Tiantian
    Zhang, Yan
    Jiang, Xiangrui
    Zhang, Leike
    Xu, Yechun
    Zhang, Yumin
    Su, Haixia
    Zhang, Qiumeng
    Shen, Jingshan
    EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2023, 257
  • [34] Exploration of SARS-CoV-2 3CLpro Inhibitors by Virtual Screening Methods, FRET Detection, and CPE Assay
    Zhao, Jun
    Ma, Qinhai
    Zhang, Baoyue
    Guo, Pengfei
    Wang, Zhe
    Liu, Yi
    Meng, Minsi
    Liu, Ailin
    Yang, Zifeng
    Du, Guanhua
    JOURNAL OF CHEMICAL INFORMATION AND MODELING, 2021, 61 (12) : 5763 - 5773
  • [35] BRET-Based Self-Cleaving Biosensors for SARS-CoV-2 3CLpro Inhibitor Discovery
    Hou, Ningke
    Peng, Chen
    Zhang, Lijing
    Zhu, Yuyao
    Hu, Qi
    MICROBIOLOGY SPECTRUM, 2022, 10 (04):
  • [36] A head-to-head comparison of the inhibitory activities of 15 peptidomimetic SARS-CoV-2 3CLpro inhibitors
    Vankadara, Subramanyam
    Wong, Yun Xuan
    Liu, Boping
    See, Yi Yang
    Tan, Li Hong
    Tan, Qian Wen
    Wang, Gang
    Karuna, Ratna
    Guo, Xue
    Tan, Shu Ting
    Fong, Jia Yi
    Joy, Joma
    Chia, C. S. Brian
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2021, 48
  • [37] In silico and in vitro assays reveal potential inhibitors against 3CLpro main protease of SARS-CoV-2
    Iype, Eldhose
    Jisha, Pillai U.
    Kumar, Indresh
    Gaastra-Nedea, Silvia V.
    Subramanian, Ramachandran
    Saha, Ranendra Narayan
    Dutta, Mainak
    JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2022, 40 (23): : 12800 - 12811
  • [38] Design and Synthesis of Michael Acceptor-Based Peptidyl β-Nitrostyrenes as 3CLpro Inhibitors of SARS-CoV-2
    Sharma, Sweta
    Yakkala, Prasanna Anjaneyulu
    Aboti, Jyoti
    Latief, Insha
    Ansari, Mairaj Ahmed
    Khan, Wajihul Hasan
    Shafi, Syed
    CHEMISTRYSELECT, 2023, 8 (24):
  • [39] Ferrocenoyl-substituted quinolinone and coumarin as organometallic inhibitors of SARS-CoV-2 3CLpro main protease
    Graf, Dominic
    Farn, Nikolas
    Klopf, Jonas
    Hojjati, Mahniya
    Schatzschneider, Ulrich
    METALLOMICS, 2023, 15 (05)
  • [40] Development of a Cell-Based Luciferase Complementation Assay for Identification of SARS-CoV-2 3CLpro Inhibitors
    Rawson, Jonathan M. O.
    Duchon, Alice
    Nikolaitchik, Olga A.
    Pathak, Vinay K.
    Hu, Wei-Shau
    VIRUSES-BASEL, 2021, 13 (02):
12345