Purification and characterization of an extracellular α-L-arabinofuranosidase from Fusarium oxysporum

被引：7
|
作者：
Christakopoulos, P [1 ]
Katapodis, P [1 ]
Hatzinikolaou, DG [1 ]
Kekos, D [1 ]
Macris, BJ [1 ]
机构：
[1] Natl Tech Univ Athens, Sector 4, Dept Chem Engn, Biotechnol Lab, Zografou 15780, Attica, Greece
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2000年 / 87卷 / 02期
关键词：
Fusarium oxysporum; alpha-L-arabinofuranosidase; purification; characterization; synergism;
D O I：
10.1385/ABAB:87:2:127
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An alpha-L-arabinofuranosidase from Fusarium oxysporum Fusarium oxysporum F3 was purified to homogeneity by a two-step ion exchange intercalated by a gel filtration chromatography. The enzyme had a molecular mass of 66 kDa and was optimally active at pH 6.0 and 60 degrees C. It hydrolyzed aryl alpha-L-arabinofuranosides and cleaved arabinosyl side chains from arabinoxylan and arabinan. There was a marked synergistic effect between the alpha-L-arabinofuranosidase and an endo-(1-->4)-beta-D-xylanase produced by F. oxysporum in the extensive hydrolysis of arabinoxylan.
引用
收藏
页码：127 / 133
页数：7
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