Nonlinear multicontrast microscopy of hematoxylin-and-eosin-stained histological sections

被引：54

作者：

Tuer, Adam ^{[1
]}

Tokarz, Danielle ^{[1
]}

Prent, Nicole ^{[1
]}

Cisek, Richard ^{[1
]}

Alami, Jennifer ^{[2
]}

Dumont, Daniel J. ^{[2
]}

Bakueva, Ludmila ^{[2
]}

Rowlands, John ^{[2
]}

Barzda, Virginijus ^{[1
]}

机构：

[1] Univ Toronto, Dept Chem & Phys Sci, Dept Phys, Inst Opt Sci, Mississauga, ON L5L 1C6, Canada

[2] Univ Toronto, Sunnybrook Hlth Sci Ctr, Dept Med Biophys, Toronto, ON M5G 2M9, Canada

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2010年 / 15卷 / 02期

基金：

加拿大创新基金会; 加拿大自然科学与工程研究理事会;

关键词：

medical imaging; multiphoton processes; nonlinear optics; nonlinear image processing; scanning microscopy; tissues; 3RD-HARMONIC GENERATION MICROSCOPY; HEMATEIN-ALUMINUM COMPLEXES; Y-MACROMOLECULE COMPLEXES; MOLECULES; CANCER;

D O I：

10.1117/1.3382908

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Imaging hematoxylin-and-eosin-stained cancerous histological sections with multicontrast nonlinear excitation fluorescence, second- and third-harmonic generation (THG) microscopy reveals cellular structures with extremely high image contrast. Absorption and fluorescence spectroscopy together with second hyperpolarizability measurements of the dyes shows that strong THG appears due to neutral hemalum aggregation and is subsequently enhanced by interaction with eosin. Additionally, fluorescence lifetime imaging microscopy reveals eosin fluorescence quenching by hemalums, showing better suitability of only eosin staining for fluorescence microscopy. Multicontrast nonlinear microscopy has the potential to differentiate between cancerous and healthy tissue at a single cell level. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3382908]

引用

页数：9

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