A simple, efficient, and economical method for recovering DNA from agarose gel

被引：2
|
作者：
Fan, CF [1 ]
Mei, XG [1 ]
机构：
[1] Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
来源：
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2005年 / 35卷 / 01期
关键词：
agarose gel; method; DNA recovery;
D O I：
10.1081/PB-200041450
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple method of recovering DNA from agarose gel that is fast. inexpensive, and friendly both to operators and environment is described. Two rows of wells are made in an agarose gel, and a DNA sample is loaded into the well nearest to the negative pole for separation by electrophoresis. Recovery is accomplished by pipetting the DNA-containing TAE buffer from the well near the positive pole after target DNA fragments have migrated into the well. A recovery rate of up to 94 +/- 2.3% was observed with this method.
引用
收藏
页码：71 / 78
页数：8
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