Application of the Taguchi method to optimize Monascus spp. culture

The Taguchi method was applied to determine optimum conditions for submerged culture of Monascus spp. fermentation to produce a high yield of monacolin K (also known as lovastain, mevinolin or mevacor). The control factors included carbon, nitrogen, oil, and salt sources and pH values. In the growth phase the optimum culture conditions are 1% whole wheat flour, 1% peptone, 0.01% olive oil and 0.01% potassium, phosphate and a pH of 5.0 (P < 0.05). In the metabolic phase the optimal culture conditions are 1% whole wheat flour 1% peptone, 0.01% soybean oil, 0.01% potassium phosphate and a pH of 3.0 (P < 0.001). Using optimal culture conditions in the growth and metabolic phases, the yield of monacolin K in the fermentation process was 151.06 ppm.