Detection of Helicobacter pylori in fecal samples of gnotobiotic mice infected with H-pylori by an immunomagnetic-bead separation technique

被引:21
|
作者
Osaki, T
Taguchi, H
Yamaguchi, H
Kamiya, S
机构
[1] Kyorin Univ, Sch Med, Dept Microbiol, Mitaka, Tokyo 181, Japan
[2] Kyorin Univ, Sch Med, Div Flow Cytometry, Mitaka, Tokyo 181, Japan
关键词
D O I
10.1128/JCM.36.1.321-323.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
By an immunomagnetic-bead (IMB) separation technique, isolation of Helicobacter pylori from gastrointestinal and fecal samples of gnotobiotic mice infected with the microorganism was tried. The isolation rate of H. pylori from stomach samples after IMB separation was not higher than that of direct culture of the samples. After IMB separation of feces, H. pylori was detectable by PCR, although H. pylori was not culturable.
引用
收藏
页码:321 / 323
页数:3
相关论文
共 50 条
  • [31] Ultrasensitive detection of H. pylori in human feces based on immunomagnetic bead capture and fluorescent quantum dots
    Chen, Lili
    Li, Xiangguang
    Zou, Tongda
    Wang, Tiantian
    Cui, Xiping
    Chen, Yingshan
    Zhang, Chunguo
    Zhao, Suqing
    ANALYST, 2019, 144 (13) : 4086 - 4092
  • [32] Use of LNA/DNA probes to improve FISH detection of H-pylori in water samples
    Kurincic, M.
    Kramar, A.
    Ambrozic, M.
    Medja, B.
    Mozina, S. Smole
    ZOONOSES AND PUBLIC HEALTH, 2007, 54 : 28 - 28
  • [33] Use of LNA/DNA probes to improve FISH detection of H-pylori in water samples
    Moreno, Y.
    Canigral, I.
    Fortuny, T.
    Alonso, J. L.
    Hernandez, J.
    Ferrus, M. A.
    ZOONOSES AND PUBLIC HEALTH, 2007, 54 : 28 - 28
  • [34] A negative Helicobacter pylori serology test is more reliable for exclusion of premalignant gastric conditions than a negative test for current H-pylori infection:: A report on histology and H-pylori detection in the general adult population
    Storskrubb, T
    Aro, P
    Ronkainen, J
    Vieth, M
    Stolte, M
    Wreiber, K
    Engstrand, L
    Nyhlin, H
    Bolling-Sternevald, E
    Talley, NJ
    Agréus, L
    SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 2005, 40 (03) : 302 - 311
  • [35] Neuro-immune interactions in the stomach of H-pylori infected mice:: Role of lymphocytes.
    Bercik, P
    Blennerhassett, PA
    Verdu, EF
    Collins, SM
    GASTROENTEROLOGY, 2001, 120 (05) : A535 - A535
  • [36] Dietary components influences the ability to recover H-pylori from infected Balb/ca mice
    Wang, X
    Sjunnesson, H
    Sturegard, E
    Wadstrom, T
    Willen, R
    Aleljung, P
    GUT, 1997, 41 : A121 - A121
  • [37] Comparison of chemiluminiscence versus lateral flow assay for the detection of Helicobacter pylori antigen in human fecal samples
    Rescalvo-Casas, Carlos
    Hernando-Gozalo, Marcos
    Pereda, Laura Seijas
    Bertolin, Carlos Garcia
    Perez-Garcia, Felipe
    Cuadros-Gonzalez, Juan
    Perez-Tanoira, Ramon
    EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2023, 42 (08) : 959 - 962
  • [38] PCR test for Helicobacter pylori detection and clarithromycin resistance prediction on fecal and bioptic samples: comparison of accuracies
    Pavoni, M.
    Beckman, E.
    Turello, G.
    Saracino, I.
    Fiorini, G.
    Saccomanno, L.
    Lazzarotto, T.
    Vaira, D.
    HELICOBACTER, 2018, 23
  • [39] PCR TEST FOR HELICOBACTER PYLORI DETECTION AND CLARITHROMYCIN RESISTANCE PREDICTION ON FECAL AND BIOPTIC SAMPLES: COMPARISON OF ACCURACIES
    Pavoni, M.
    Turello, G.
    Saracino, I. M.
    Fiorini, G.
    Saccomanno, L.
    Lazzarotto, T.
    Vaira, B.
    Beckman, E.
    DIGESTIVE AND LIVER DISEASE, 2019, 51 : E106 - E107
  • [40] H-pylori infected mucosa in gastric ulcer samples:: Phospholipase alcohol dehydrogenase and urease activities.
    Barreto-Zuñiga, R
    Okuyama, M
    Kato, Y
    Marotta, F
    Ohta, H
    Takekoshi, T
    Maruyama, M
    Murguia, D
    GASTROENTEROLOGY, 1998, 114 (04) : A67 - A67