Circular RNA circPVT1 Contributes to Doxorubicin (DXR) Resistance of Osteosarcoma Cells by Regulating TRIAP1 via miR-137

被引:25
|
作者
Li, Dan [1 ]
Huang, Yan [2 ]
Wang, Gang [2 ]
机构
[1] First Hosp Jilin Univ, Dept Radiol, Changchun, Jilin, Peoples R China
[2] China Japan Union Hosp Jilin Univ, Dept Orthoped, Changchun, Jilin, Peoples R China
关键词
MULTIDRUG-RESISTANCE; SENSITIZES OSTEOSARCOMA; BREAST-CANCER; INHIBITION; CISPLATIN; APOPTOSIS; TRANSPORTERS; METASTASIS; AXIS;
D O I
10.1155/2021/7463867
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background. Chemoresistance is a major obstacle to the treatment of osteosarcoma patients. Circular RNA (circRNA) circPVT1 has been reported to be related to the doxorubicin (DXR) resistance in osteosarcoma. This study is designed to explore the role and mechanism of circPVT1 in the DXR resistance of osteosarcoma. Methods. circPVT1, microRNA-137 (miR-137), and TP53-regulated inhibitor of apoptosis 1 (TRIAP1) levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR). The protein levels of ATP-binding cassette, subfamily C, member 1 (ABCC1), multidrug resistance-associated protein 1 (MRP-1), cleaved- (c-) caspase-3, B-cell lymphoma-2 (Bcl-2), and TRIAP1 were examined by a western blot assay. Cell viability, proliferation, and apoptosis were detected by cell counting kit-8 (CCK-8), colony formation, and flow cytometry assays, severally. The binding relationship between miR-137 and circPVT1 or TRIAP1 was predicted by starbase 3.0 and then verified by dual-luciferase reporter and RNA Immunoprecipitation (RIP) assays. The biological role of circPVT1 in osteosarcoma tumor growth and drug resistance was examined by the xenograft tumor model in vivo. Results. circPVT1 and TRIAP1 were highly expressed, and miR-137 was decreased in DXR-resistant osteosarcoma tissues and cells. Moreover, circPVT1 knockdown could boost DXR sensitivity by inhibiting DXR-caused proliferation and DXR-induced apoptosis in DXR-resistant osteosarcoma cells in vitro. The mechanical analysis discovered that circPVT1 acted as a sponge of miR-137 to regulate TRIAP1 expression. circPVT1 silencing increased the drug sensitivity of osteosarcoma in vivo. Conclusion. circPVT1 boosted DXR resistance of osteosarcoma cells partly by regulating the miR-137/TRIAP1 axis, hinting a promising therapeutic target for the osteosarcoma treatment.
引用
收藏
页数:19
相关论文
共 50 条
  • [21] CircPVT1 promotes the tumorigenesis and metastasis of osteosarcoma via mediation of miR-26b-5p/CCNB1 axis (vol 40, pg 581, 2022)
    Huang, Sheng-Xiang
    Mei, Hai-Bo
    Liu, Kun
    Tang, Jin
    Wu, Jiang-Yan
    Zhu, Guang-Hui
    Ye, Wei-Hua
    JOURNAL OF BONE AND MINERAL METABOLISM, 2024, 42 (05) : 618 - 618
  • [22] Circular RNA circHECTD1 prevents Diosbulbin-B-sensitivity via miR-137/PBX3 axis in gastric cancer
    Lu, Yizhuo
    Li, Long
    Li, Lianghui
    Wu, Guoyang
    Liu, Guoyan
    CANCER CELL INTERNATIONAL, 2021, 21 (01)
  • [23] Regulation of Apoptosis and Inflammatory Response in Interleukin-1β-Induced Nucleus Pulposus Cells by miR-125b-5p Via Targeting TRIAP1
    Jie, Jian
    Xu, Xiaoming
    Li, Weilin
    Wang, Guihua
    BIOCHEMICAL GENETICS, 2021, 59 (02) : 475 - 490
  • [24] Regulation of Apoptosis and Inflammatory Response in Interleukin-1β-Induced Nucleus Pulposus Cells by miR-125b-5p Via Targeting TRIAP1
    Jian Jie
    Xiaoming Xu
    Weilin Li
    Guihua Wang
    Biochemical Genetics, 2021, 59 : 475 - 490
  • [25] NCK1-AS1 Increases Drug Resistance of Glioma Cells to Temozolomide by Modulating miR-137/TRIM24
    Chen, Mingsheng
    Cheng, Yingying
    Yuan, Zhihai
    Wang, Fenglu
    Yang, Lei
    Zhao, Haikang
    CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS, 2020, 35 (02) : 101 - 108
  • [26] Circular RNA circDOCK1 contributes to osteosarcoma progression by acting as a ceRNA for miR-936 to regulate LEF1
    Xu, Gang
    Zhang, Haijiao
    Shi, Yuxia
    Yang, Fan
    JOURNAL OF BONE ONCOLOGY, 2022, 36
  • [27] Long Noncoding RNA Small Nucleolar RNA Host Gene 1 (SNHG1) Promotes Renal Cell Carcinoma Progression and Metastasis by Negatively Regulating miR-137
    Zhao, Shiyue
    Wang, Yangwei
    Luo, Manyu
    Cui, Wenpeng
    Zhou, Xiaoxi
    Miao, Lining
    MEDICAL SCIENCE MONITOR, 2018, 24 : 3824 - 3831
  • [28] Long noncoding RNA ZFPM2-AS1 promotes the tumorigenesis of renal cell cancer via targeting miR-137
    Liu, J. -G.
    Wang, H. -B.
    Wan, G.
    Yang, M. -Z.
    Jiang, X. -J.
    Yang, J. -Y.
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2020, 24 (18) : 9238 - 9238
  • [29] circ_0002060 Enhances Doxorubicin Resistance in Osteosarcoma by Regulating the miR-198/ABCB1 Axis
    Ji, Yuan
    Liu, Jun
    Zhu, Wenshuai
    Ji, Jianqin
    CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS, 2023, 38 (09) : 585 - 595
  • [30] Long noncoding RNA SNHG12 mediates doxorubicin resistance of osteosarcoma via miR-320a/MCL1 axis
    Zhou, Binghua
    Li, Lijun
    Li, Yajing
    Sun, Huimin
    Zeng, Changchun
    BIOMEDICINE & PHARMACOTHERAPY, 2018, 106 : 850 - 857