This study investigated the quality of washed and unwashed eggs stored at 7 degrees C (WC and UC for washed and unwashed eggs, respectively) and 25 degrees C (WR and UR for washed and unwashed eggs, respectively) for 4 weeks. The results show that the Haugh unit, albumin pH, thick albumin ratio, yolk index, air cell size, and S-ovalbumin content of UC were significantly the most superior, followed by those of WC, WR, and UR, in that order. Scanning electron microscopy and cuticle staining confirmed the damages and decreased cuticle coverage caused by washing and extended storage. Attenuated total reflection Fourier transform infrared spectroscopy revealed that cuticle composition changed significantly after washing and storage (P < 0.05). High correlations were observed between the quality parameters evaluated. In conclusion, storage temperature critically influenced egg quality, and egg washing reduced cuticle coverage. (C) 2016 Elsevier Ltd. All rights reserved.