Axial resolution enhancement in light sheet microscopy using mode modulation

被引：0
|
作者：
Zhang, Chengfeng [1 ]
Chen, Yuchen [1 ]
Kuang, Cuifang [1 ,2 ,3 ]
Liu, Xu [1 ,2 ,3 ]
机构：
[1] Zhejiang Univ, Coll Opt Sci & Engn, State Key Lab Modern Opt Instrumentat, Hangzhou 310027, Zhejiang, Peoples R China
[2] Zhejiang Univ, Ningbo Res Inst, Ningbo 315100, Peoples R China
[3] Shanxi Univ, Collaborat Innovat Ctr Extreme Opt, Taiyuan 030006, Peoples R China
来源：
ADVANCED OPTICAL IMAGING TECHNOLOGIES II | 2019年 / 11186卷
基金：
中国国家自然科学基金;
关键词：
light sheet fluorescence microscopy; super-resolution microscopy; nonlinear effect; mode modulation; DIFFRACTION LIMIT; SUPERRESOLUTION;
D O I：
10.1117/12.2537528
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Light sheet fluorescence microscopy (LSFM) is widely used in biological imaging because of its low photobleaching and phototoxicity. The axial resolution of LSFM is determined and also limited by the thickness of the light sheet and the numerical aperture (NA) of the detection objective. We propose a novel method, light sheet modulation fluorescence microscopy (LSMFM) which is able to achieve a promising axial resolution enhancement of light sheet microscopy.
引用
收藏
页数：7
相关论文
共 50 条
  • [31] Photoswitching-Enabled Contrast Enhancement in Light Sheet Fluorescence Microscopy
    Vettenburg, Tom
    Corral, Angelica
    Rodriguez-Pulido, Alberto
    Flors, Cristina
    Ripoll, Jorge
    ACS PHOTONICS, 2017, 4 (03): : 424 - 428
  • [32] Light sheet microscopy
    Weber, Michael
    Mickoleit, Michaela
    Huisken, Jan
    QUANTITATIVE IMAGING IN CELL BIOLOGY, 2014, 123 : 193 - 215
  • [33] High axial resolution and long field of view for light-sheet fluorescence microscopy via double-beam aperture
    L V Nhu
    Xuanhoi Hoang
    Minhnghia Pham
    Hoanghai Le
    EUROPEAN PHYSICAL JOURNAL PLUS, 2020, 135 (06):
  • [34] High axial resolution and long field of view for light-sheet fluorescence microscopy via double-beam aperture
    L. V. Nhu
    Xuanhoi Hoang
    Minhnghia Pham
    Hoanghai Le
    The European Physical Journal Plus, 135
  • [35] Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution
    Chakraborty, Tonmoy
    Driscoll, Meghan K.
    Jeffery, Elise
    Murphy, Malea M.
    Roudot, Philippe
    Chang, Bo-Jui
    Vora, Saumya
    Wong, Wen Mai
    Nielson, Cara D.
    Zhang, Hua
    Zhemkov, Vladimir
    Hiremath, Chitkale
    De la Cruz, Estanislao Daniel
    Yi, Yating
    Bezprozvanny, Ilya
    Zhao, Hu
    Tomer, Raju
    Heintzmann, Rainer
    Meeks, Julian P.
    Marciano, Denise K.
    Morrison, Sean J.
    Danuser, Gaudenz
    Dean, Kevin M.
    Fiolka, Reto
    NATURE METHODS, 2019, 16 (11) : 1109 - +
  • [36] An AutoPilot platform for high-resolution light-sheet microscopy
    Graham, Dustin M.
    LAB ANIMAL, 2017, 46 (02) : 25 - 25
  • [37] Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution
    Tonmoy Chakraborty
    Meghan K. Driscoll
    Elise Jeffery
    Malea M. Murphy
    Philippe Roudot
    Bo-Jui Chang
    Saumya Vora
    Wen Mai Wong
    Cara D. Nielson
    Hua Zhang
    Vladimir Zhemkov
    Chitkale Hiremath
    Estanislao Daniel De La Cruz
    Yating Yi
    Ilya Bezprozvanny
    Hu Zhao
    Raju Tomer
    Rainer Heintzmann
    Julian P. Meeks
    Denise K. Marciano
    Sean J. Morrison
    Gaudenz Danuser
    Kevin M. Dean
    Reto Fiolka
    Nature Methods, 2019, 16 : 1109 - 1113
  • [38] An AutoPilot platform for high-resolution light-sheet microscopy
    Dustin M. Graham
    Lab Animal, 2017, 46 : 25 - 25
  • [39] The Lateral and Axial Localization Uncertainty in Super-Resolution Light Microscopy
    Rieger, Bernd
    Stallinga, Sjoerd
    CHEMPHYSCHEM, 2014, 15 (04) : 664 - 670
  • [40] Light-sheet microscopy using an Airy beam
    Vettenburg, Tom
    Dalgarno, Heather I. C.
    Nylk, Jonathan
    Coll-Llado, Clara
    Ferrier, David E. K.
    Cizmar, Tomas
    Gunn-Moore, Frank J.
    Dholakia, Kishan
    NATURE METHODS, 2014, 11 (05) : 541 - 544
12345