Axial resolution enhancement in light sheet microscopy using mode modulation

被引：0
|
作者：
Zhang, Chengfeng [1 ]
Chen, Yuchen [1 ]
Kuang, Cuifang [1 ,2 ,3 ]
Liu, Xu [1 ,2 ,3 ]
机构：
[1] Zhejiang Univ, Coll Opt Sci & Engn, State Key Lab Modern Opt Instrumentat, Hangzhou 310027, Zhejiang, Peoples R China
[2] Zhejiang Univ, Ningbo Res Inst, Ningbo 315100, Peoples R China
[3] Shanxi Univ, Collaborat Innovat Ctr Extreme Opt, Taiyuan 030006, Peoples R China
来源：
ADVANCED OPTICAL IMAGING TECHNOLOGIES II | 2019年 / 11186卷
基金：
中国国家自然科学基金;
关键词：
light sheet fluorescence microscopy; super-resolution microscopy; nonlinear effect; mode modulation; DIFFRACTION LIMIT; SUPERRESOLUTION;
D O I：
10.1117/12.2537528
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Light sheet fluorescence microscopy (LSFM) is widely used in biological imaging because of its low photobleaching and phototoxicity. The axial resolution of LSFM is determined and also limited by the thickness of the light sheet and the numerical aperture (NA) of the detection objective. We propose a novel method, light sheet modulation fluorescence microscopy (LSMFM) which is able to achieve a promising axial resolution enhancement of light sheet microscopy.
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收藏
页数：7
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