Study of the effect of simulated space environment on nucleoprotein and DNA thin films

被引：0

作者：

Fekete, A ^{[1
]}

Módos, K ^{[1
]}

Hegedüs, M ^{[1
]}

Rontó, G ^{[1
]}

Kovács, G ^{[1
]}

Bérces, A ^{[1
]}

Kargl, G ^{[1
]}

Kömle, NI ^{[1
]}

Lammer, H ^{[1
]}

机构：

[1] Semmelweis Univ, Inst Biophys & Radiat Biol, H-1088 Budapest, Hungary

来源：

PROCEEDINGS OF THE SECOND EUROPEAN WORKSHOP ON EXO-ASTROBIOLOGY | 2002年 / 518卷

关键词：

D O I：

暂无

中图分类号：

P1 [天文学];

学科分类号：

0704 ;

摘要：

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy. To provide experimental evidence for the hypothesis that interplanetary transfer of life is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (lambda = 254 nm) and high vacuum (10(-5) mbar). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.

引用

页码：67 / 70

页数：4

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