Detection of peanut (Arachis hypogaea) allergen by Real-time PCR method with internal amplification control

被引：29
|
作者：
Zhang, Wen-Ju [1 ]
Cai, Qin [1 ]
Guan, Xiao [1 ]
Chen, Qin [1 ]
机构：
[1] Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai, Peoples R China
来源：
FOOD CHEMISTRY | 2015年 / 174卷
基金：
中国国家自然科学基金;
关键词：
Peanut (Arachis hypogaea); Allergen; Ara h 1; Real-time PCR; Internal amplification control (IAC); POLYMERASE-CHAIN-REACTION; ANAPHYLACTIC REACTIONS; PROCESSED FOODS; DIAGNOSTIC PCR; TRACE AMOUNTS; DNA; FATALITIES; DESIGN; ASSAYS;
D O I：
10.1016/j.foodchem.2014.11.091
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Specific primer sets were designed based on the DNA sequence of Ara h I, one of the major peanut (Arachis hypogaea) allergens, and a competitive internal amplification control (IAC) was designed by compound primer technology. By choosing 314 copies/PCR as the IAC dosage, a Real-time PCR method with IAC was established for detecting peanut allergen Ara h 1 DNA. The method showed high specificity with a detection limit of 0.005% peanut. A series of commercial food products with/without peanut components were tested. Among these products, the peanut allergen Ara h 1 DNA could be detected in 12 products labelled containing peanut ingredients, in two without a declaration of peanut and one labelled that was produced in a facility that produced peanut-containing foods. This indicates that the method is highly sensitive for the detection of peanut ingredients in foods. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：547 / 552
页数：6
相关论文
共 50 条
  • [11] Real-time PCR assay with an endogenous internal amplification control for detection and quantification of Anaplasma marginale in bovine blood
    Kovalchuk, Svetlana N.
    Babii, Anna, V
    Arkhipova, Anna L.
    TICKS AND TICK-BORNE DISEASES, 2020, 11 (02)
  • [12] A real-time PCR for detection of pathogenic Yersinia enterocolitica in food combined with an universal internal amplification control system
    Maede, D.
    Reiting, R.
    Strauch, E.
    Ketteritzsch, K.
    Wicke, A.
    JOURNAL FUR VERBRAUCHERSCHUTZ UND LEBENSMITTELSICHERHEIT-JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY, 2008, 3 (02): : 141 - 151
  • [13] TaqMan real-time RT-PCR assay with an internal amplification control for rapid detection of Bluetongue virus
    Chew, Chaolin
    Han, Diangang
    Li, Jing
    Ye, Lingling
    Ji, Xincheng
    Nie, Fuping
    Song, Zhigang
    Luo, Qianmin
    Shi, Yaling
    Pu, Qiqi
    Ai, Jun
    Xin, Jige
    MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE, 2024, 80 (02): : 68 - 75
  • [14] A real-time PCR for Detection of Pathogenic Yersinia enterocolitica in food combined with an Universal Internal Amplification Control System
    Dietrich Mäde
    R. Reiting
    E. Strauch
    K. Ketteritzsch
    A. Wicke
    Journal für Verbraucherschutz und Lebensmittelsicherheit, 2008, 3 : 141 - 151
  • [15] Real-time PCR with internal amplification control for the detection of Cronobacter spp. (Enterobacter sakazakii) in food samples
    Wang, Xiang
    Zhu, Changqing
    Xu, Xinglian
    Zhou, Guanghong
    FOOD CONTROL, 2012, 25 (01) : 144 - 149
  • [16] Quantitative real-time PCR method with internal amplification control to quantify cyclopiazonic acid producing molds in foods
    Rodriguez, Alicia
    Werning, Maria L.
    Rodriguez, Mar
    Bermudez, Elena
    Cordoba, Juan J.
    FOOD MICROBIOLOGY, 2012, 32 (02) : 397 - 405
  • [17] ELIMINATION OF PCR INHIBITORS IN ROUTINE DIAGNOSTIC REAL-TIME PCR ASSAY AND RESULTS OF INTERNAL AMPLIFICATION CONTROL
    Sayan, Murat
    Meric, Meliha
    Celebi, Suna
    Willke, Ayse
    MIKROBIYOLOJI BULTENI, 2009, 43 (01): : 179 - 181
  • [18] Duplex loop-mediated isothermal amplification assay for peanut (Arachis hypogaea) and almond (Prunus dulcis) detection of allergen coding genes
    Kim, Hong-Rae
    Suh, Seung-Man
    Kang, Hyeon-Bee
    Shin, So-Won
    Kim, Hae-Yeong
    FOOD CONTROL, 2022, 138
  • [19] Fast Real-Time PCR for the Detection of Crustacean Allergen in Foods
    Herrero, Beatriz
    Vieites, Juan M.
    Espineira, Montserrat
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, 60 (08) : 1893 - 1897
  • [20] Rapid detection of Cronobacter sakazakii by real-time PCR based on the cgcA gene and TaqMan probe with internal amplification control
    Hu, Shuangfang
    Yu, Yigang
    Li, Rong
    Wu, Xinwei
    Xiao, Xinglong
    Wu, Hui
    CANADIAN JOURNAL OF MICROBIOLOGY, 2016, 62 (03) : 191 - 200
12345