Detection of peanut (Arachis hypogaea) allergen by Real-time PCR method with internal amplification control

被引：29
|
作者：
Zhang, Wen-Ju [1 ]
Cai, Qin [1 ]
Guan, Xiao [1 ]
Chen, Qin [1 ]
机构：
[1] Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai, Peoples R China
来源：
FOOD CHEMISTRY | 2015年 / 174卷
基金：
中国国家自然科学基金;
关键词：
Peanut (Arachis hypogaea); Allergen; Ara h 1; Real-time PCR; Internal amplification control (IAC); POLYMERASE-CHAIN-REACTION; ANAPHYLACTIC REACTIONS; PROCESSED FOODS; DIAGNOSTIC PCR; TRACE AMOUNTS; DNA; FATALITIES; DESIGN; ASSAYS;
D O I：
10.1016/j.foodchem.2014.11.091
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Specific primer sets were designed based on the DNA sequence of Ara h I, one of the major peanut (Arachis hypogaea) allergens, and a competitive internal amplification control (IAC) was designed by compound primer technology. By choosing 314 copies/PCR as the IAC dosage, a Real-time PCR method with IAC was established for detecting peanut allergen Ara h 1 DNA. The method showed high specificity with a detection limit of 0.005% peanut. A series of commercial food products with/without peanut components were tested. Among these products, the peanut allergen Ara h 1 DNA could be detected in 12 products labelled containing peanut ingredients, in two without a declaration of peanut and one labelled that was produced in a facility that produced peanut-containing foods. This indicates that the method is highly sensitive for the detection of peanut ingredients in foods. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：547 / 552
页数：6
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