Establishment of human hepatocellular carcinoma CAM xenograft model and observation of angiogenesis characteristics

被引：0
|
作者：
Lv, Xiang [1 ]
Ling, Li [2 ]
Xia, Ying [1 ]
Xu, Yan-Feng [1 ]
Hou, Feng-Gang [1 ]
Ren, Jian-Lin [1 ]
Zhang, Xiao-Feng [1 ]
Li, Yan [1 ]
机构：
[1] Shanghai Univ Tradit Chinese Med, Shanghai Municipal Hosp Tradit Chinese Med, Shanghai 200071, Peoples R China
[2] Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE | 2016年 / 9卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Hepatoma; chick chorioallantoic membrane; models; animal; angiogenesis; EMBRYO CHORIOALLANTOIC MEMBRANE;
D O I：
暂无
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
To investigate the establishing method of human hepatoma cell line Bel-7402 CAM transplantation model and to observe and analyze the characteristics of angiogenesis. Different concentrations of human hepatoma Bel-7402 cells were inoculated on chick embryo chorioallantoic membrane (CAM) to observe CAM tumor survival factors, tumor growth characteristics and angiogenesis. Human hepatocellular carcinoma CAM xenograft model was established. In the model, tumor was easy to grow, with a strong angiogenic effect. The model is easy to copy, and can dynamically observe HCC angiogenesis process, which can be used to study the biological behavior of HCC and screen drugs.
引用
收藏
页码：3698 / 3703
页数：6
相关论文
共 50 条
  • [41] Development of a Mouse Angiogenesis Xenograft Model Utilizing Human Endothelial Cells
    Wilkie, D. P.
    JOURNAL OF THE AMERICAN ASSOCIATION FOR LABORATORY ANIMAL SCIENCE, 2010, 49 (05): : 738 - 738
  • [42] Establishment and initial characterization of human hepatocellular carcinoma organoid culture
    Scholch, Sebastian
    Nanduri, Lahiri K.
    Kuhn, Daniel
    Weitz, Jurgen
    Koch, Moritz
    Rahbari, Nuh N.
    CANCER RESEARCH, 2016, 76
  • [43] Establishment and characterization of human metastatic hepatocellular carcinoma cell line
    Wen, JM
    Huang, JF
    Hu, LA
    Wang, WS
    Zhang, M
    Sham, JST
    Xu, JM
    Zeng, WF
    Xie, D
    Liang, LJ
    Guan, XY
    CANCER GENETICS AND CYTOGENETICS, 2002, 135 (01) : 91 - 95
  • [44] Establishment of a predictive patient-derived xenograft model for renal cell carcinoma
    Di Martino, Simona
    De Luca, Gabriele
    Grassi, Ludovica
    Federici, Giulia
    De Salvo, Laura
    Di Pace, Anna Laura
    Addario, Antonio
    Muto, Giovanni
    Costantini, Manuela
    Biffoni, Mauro
    Signore, Michele
    Sentinelli, Steno
    Milella, Michele
    Gallucci, Michele
    Bonci, Desiree
    De Maria, Ruggero
    CANCER RESEARCH, 2016, 76
  • [45] Establishment and characterization of a primary human chordoma xenograft model Laboratory investigation
    Siu, I-Mei
    Salmasi, Vafi
    Orr, Brent A.
    Zhao, Qi
    Binder, Zev A.
    Tran, Christine
    Ishii, Masaru
    Riggins, Gregory J.
    Hann, Christine L.
    Gallia, Gary L.
    JOURNAL OF NEUROSURGERY, 2012, 116 (04) : 801 - 809
  • [46] ESTABLISHMENT OF AND PROOF OF CONCEPT FOR A HUMAN PEDIATRIC BRAINSTEM GLIOMA XENOGRAFT MODEL
    Kuan, Chien-Tsun
    Li, Jianjun
    Chandramohan, Vidyalakshmi
    Chang, Jinli
    Bigner, Darell
    NEURO-ONCOLOGY, 2011, 13 : I7 - I7
  • [47] Establishment of a Novel Xenograft Model for Human Uterine Leiomyoma in Immunodeficient Mice
    Tsuiji, Kenji
    Takeda, Takashi
    Li, Bin
    Kondo, Akiko
    Ito, Mamoru
    Yaegashi, Nobuo
    TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 2010, 222 (01): : 55 - 61
  • [48] Establishment of a pattern recognition metabolomics model for the diagnosis of hepatocellular carcinoma
    Peng-Cheng Zhou
    Lun-Quan Sun
    Li Shao
    Lun-Zhao Yi
    Ning Li
    Xue-Gong Fan
    World Journal of Gastroenterology, 2020, (31) : 4607 - 4623
  • [49] Establishment of an in vivo highly metastatic rat hepatocellular carcinoma model
    Futakuchi, M
    Hirose, M
    Ogiso, T
    Kato, K
    Sano, M
    Ogawa, K
    Shirai, T
    JAPANESE JOURNAL OF CANCER RESEARCH, 1999, 90 (11): : 1196 - 1202
  • [50] A NEW MODEL OF ORTHOTOPIC HUMAN PANCREATIC CARCINOMA XENOGRAFT
    Porrero-Guerrero, B.
    Lopez-Arevalo, C. A.
    Fernando-Macias, E.
    Ugalde-Serrano, P.
    Garcia-Perez, E.
    Suarez-Fernandez, L.
    Galvan-Hernandez, J. A.
    Vazquez-Villa, J. F.
    Castro-Santos, P.
    de los Toyos-Gonzalez, J. R.
    Gonzalez del Rey, M. C.
    Menendez-Rodriguez, M. P.
    Garcia-Pravia, C.
    Barneo, L.
    BRITISH JOURNAL OF SURGERY, 2013, 100 : 7 - 7
12345