Anthelmintic activity of Leucaena leucocephala pod on gastrointestinal nematodes of sheep (in vitro)

Due to the resistance that parasites have acquired to conventional drugs, new strategies are currently being sought to control the cycle of parasites associated with health problems in animal production. The objective of the study was to determine the anthelmintic activity of Leucaena leucocephala pod (LLP) hydroalcoholic extract on gastrointestinal nematodes of sheep. A hydroalcoholic extract of LLP was prepared and the concentrations 50, 25, 12.5 and 6.25 mg/mL were evaluated to determine the activity on eggs hatching inhibition of gastrointestinal nematodes in sheep and the mortality on larvae of nematodes (L3). It was determined that the hydroalcoholic extract of LLP at 50 mg / mL inhibited the eggs hatching of nematode (20%) and at 6.25 mg/mL killed 22% of the larvae of nematodes (L3). The hydroalcoholic extract of LLP contains active metabolites that act in inhibition eggs hatching on field nematodes and on infective L3. The hydroalcoholic extract of LLP can be an unconventional alternative for the prevention and control of parasites in small ruminant at field level.