CaCO3-Chitosan Composites Granules for Instant Hemostasis and Wound Healing

Excessive bleeding induces a high risk of death and is a leading cause of deaths that result from traffic accidents and military conflict. In this paper, we developed a novel porous chitosan-CaCO3 (CS-CaCO3) composite material and investigated its hemostatic properties and wound healing performance. The CS-CaCO3 composites material was prepared via a wet-granulation method. Granulation increases the infiltrating ability of the CS-CaCO3 composites material. The improved water absorption ability was enhanced to 460% for the CS-CaCO3 composites material compared to the CaCO3 or chitosan with only one single component. The coagulation studies in vivo illustrated that the blood clotting time was greatly reduced from 31 s for CaCO3 to 16 s for the CS-CaCO3 composite material. According to the results of the wound healing experiments in rats, it was found that the CS-CaCO3 composite material can promote wound healing. The CS-CaCO3 composite material could accelerate wound healing to a rate of 9 days, compared with 12 days for the CaCO3. The hemostatic activity, biocompatibility, and low cost of CS-CaCO3 composite material make it a potential agent for effective hemostatic and wound healing materials.