Ti3C2 nanosheets with broad-spectrum antioxidant activity for cytoprotection against oxidative stress

Redox regulation in biological systems represents a fascinating method for treatment and prevention of oxidative stress induced diseases. The key and difficult point is to find ideal materials with excellent antioxidant capability and good biocompatibility. To this end, ultra-thin two-dimensional MXene (Ti3C2) nanosheets (NSs) were investigated for their antioxidant capability. It is found that Ti3C2 NSs can scavenge efficiently reactive oxygen and nitrogen species ((OH)-O-center dot, H2O2, O-2(-center dot) and (NO)-N-center dot), ABTS(+center dot) and DPPH center dot free radicals in a concentration dependent manner, showing broad-spectrum antioxidant activities. Ti3C2 NSs exhibit higher antioxidant activity and broader antioxidant capability than natural antioxidant molecules. The significant role of PEG modified Ti3C2 with good stability in preventing cell damage against oxidative stress was demonstrated. Upon treatment of H2O2 induced oxidative stress with Ti3C2, the intracellular ROS level decreases and the cell survival rate increases significantly. An antioxidant mechanism based on gradient oxidation was proposed to account for the superior antioxidant activity of Ti3C2. Our result proves that ultra-thin MXenes as antioxidants have great potential in preventing oxidative stress caused biological damage.