Multiplex detection of histone-modifying enzymes by total internal reflection fluorescence-based single-molecule detection

被引：49
|
作者：
Ma, Fei [1 ,2 ]
Liu, Meng [1 ]
Wang, Zi-yue [1 ]
Zhang, Chun-yang [1 ,2 ]
机构：
[1] Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Collaborat Innovat Ctr Functionalized Probes Chem, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Peoples R China
[2] Chinese Acad Sci, Shenzhen Inst Adv Technol, Single Mol Detect & Imaging Lab, Shenzhen 518055, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 06期
基金：
中国国家自然科学基金;
关键词：
PROTEIN-PHOSPHORYLATION; DNA-REPLICATION; ASSAY; ACETYLATION; METHYLTRANSFERASES; GCN5; H3; ACETYLTRANSFERASES; SPECIFICITY; COMPLEXES;
D O I：
10.1039/c5cc08797j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We develop a sensitive and selective method for the multiplex detection of histone-modifying enzymes (HMEs) through the integration of antibody-based fluorescence labeling with total internal reflection fluorescence (TIRF)-based single-molecule detection. This method exhibits excellent specificity and high sensitivity with a detection limit of 21 pM for histone acetyltransferase GcN5 and 12 pM for histone methyltransferase G9a, and it can be applied for the screening of HME inhibitors as well.
引用
收藏
页码：1218 / 1221
页数：4
相关论文
共 50 条
  • [41] Theoretical investigation of aspects of single-molecule fluorescence detection in microcapillaries
    Enderlein, J
    CYTOMETRY, 1999, 36 (03): : 195 - 199
  • [42] Single-Molecule Fluorescence Detection of a Synthetic Heparan Sulfate Disaccharide
    Dalton, Charlotte E.
    Quinn, Steven D.
    Rafferty, Aidan
    Morten, Michael J.
    Gardiner, John M.
    Magennis, Steven W.
    CHEMPHYSCHEM, 2016, 17 (21) : 3442 - 3446
  • [43] LASER-INDUCED FLUORESCENCE DETECTION OF A SINGLE-MOLECULE IN A CAPILLARY
    LEE, YH
    MAUS, RG
    SMITH, BW
    WINEFORDNER, JD
    ANALYTICAL CHEMISTRY, 1994, 66 (23) : 4142 - 4149
  • [44] Single-molecule detection by two-photon excitation of fluorescence
    Zander, C
    Brand, L
    Eggeling, C
    Drexhage, KH
    Seidel, CAM
    ADVANCES IN FLUORESCENCE SENSING TECHNOLOGY III, 1997, 2980 : 552 - 558
  • [45] Single-molecule detection in liquids by laser-induced fluorescence
    Goodwin, PM
    Ambrose, WP
    Keller, RA
    ACCOUNTS OF CHEMICAL RESEARCH, 1996, 29 (12) : 607 - 613
  • [46] Single-molecule identification by spectrally and time resolved fluorescence detection
    Prummer, M
    Hübner, CG
    Sick, B
    Hecht, B
    Renn, A
    Wild, UP
    ANALYTICAL CHEMISTRY, 2000, 72 (03) : 443 - 447
  • [47] Single-molecule detection by two-photon-excited fluorescence
    Mertz, J
    Xu, C
    Webb, WW
    OPTICS LETTERS, 1995, 20 (24) : 2532 - 2534
  • [48] Single-molecule fluorescence-based studies on the dynamics, assembly and catalytic mechanism of the spliceosome
    Warnasooriya, Chandani
    Rueda, David
    BIOCHEMICAL SOCIETY TRANSACTIONS, 2014, 42 : 1211 - 1218
  • [49] Analytical epigenetics: single-molecule optical detection of DNA and histone modifications
    Heck, Christian
    Michaeli, Yael
    Bald, Ilko
    Ebenstein, Yuval
    CURRENT OPINION IN BIOTECHNOLOGY, 2019, 55 : 151 - 158
  • [50] Single-molecule interaction between circularly permuted green fluorescent protein and trypsin by total internal reflection fluorescence microscopy
    Wang, Tong
    Isoshima, Takashi
    Miyawaki, Atsushi
    Hara, Masahiko
    COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2006, 284 (395-400) : 395 - 400
12345