In this article the results of the research focused on the calorific value capacity in the dry matter of above-ground biomass of beech, sessile oak and hornbeam are presented. For each tree species, 5-6 trees were felled and biomass samples were taken. From the lower, middle and crown part of the trunks, a disc of wood with bark was cut off and also a sample of branches (small-wood) was taken from crowns. On the discs, the bark was separated from the wood. The calorific value content was determined from all samples and fractions (wood, bark, small-wood). The differences in the calorific value among the fractions, their position on the trunk and tree species were investigated by an analysis of variance and a t-test. The biggest differences are between wood and bark. The bark has lower values of calorific value than wood, by 1,000-1,700 J g(-1). Coniferous tree species have higher calorific value by 600-900 J g(-1) (3-4%) for wood; 1,000-1,400 J g(-1) (5-7%) for small-wood and 2,400-2,700 J g(-1) (13-15%) for bark compared to deciduous species.