Effects of nitriles and amides on the growth and nitrile hydratase activity of the Rhodococcus sp strain gt1

被引：11

作者：

Maksimov, AY ^{[1
]}

Kuznetsova, MV ^{[1
]}

Ovechkina, GV ^{[1
]}

Kozlov, SV ^{[1
]}

Maksimova, YG ^{[1
]}

Demakov, VA ^{[1
]}

机构：

[1] Russian Acad Sci, Ural Div, Inst Ecol & Genet Microorganisms, Perm 614081, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2003年 / 39卷 / 01期

关键词：

D O I：

10.1023/A:1021798010261

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Effects of some nitriles and amides, as well as glucose and ammonium, on the growth and the nitrile hydratase (EC 4.2.1.84) activity of the Rhodococcus sp. strain gt1 isolated from soil were studied. The activity of nitrile hydratase mainly depended on the carbon and nitrogen supply to cells. The activity of nitrile hydratase was high in the presence of glucose and ammonium at medium concentrations and decreased at concentrations of glucose of more than 0.3%. Saturated unsubstituted aliphatic nitriles and amides were found to be a good source of nitrogen and carbon. However, the presence of nitriles and amides in the medium was not absolutely necessary for the expression of the activity of nitrile hydratase of the Rhodococcus sp. strain gt1.

引用

页码：55 / 59

页数：5

共 38 条

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