Host Factor Nucleoporin 93 Is Involved in the Nuclear Export of Influenza Virus RNA

被引：9
|
作者：
Furusawa, Yuri [1 ]
Yamada, Shinya [1 ]
Kawaoka, Yoshihiro [1 ,2 ,3 ]
机构：
[1] Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol, Div Virol, Tokyo, Japan
[2] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, Madison, WI 53706 USA
[3] Univ Tokyo, Dept Special Pathogens, Int Res Ctr Infect Dis, Inst Med Sci, Tokyo, Japan
来源：
FRONTIERS IN MICROBIOLOGY | 2018年 / 9卷
关键词：
influenza A virus; host factor; Nucleoporin; 93; nuclear export; A549; A VIRUSES; REPLICATION; COMPLEX; PROTEIN; BINDING; SCREEN; CELLS; NUP93; ASSAY;
D O I：
10.3389/fmicb.2018.01675
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Influenza virus replication relies on the functions of host factors. In our previous study, we identified host factors involved in virus replication and began analyses of their roles in this process. In this study, we focused on Nucleoporin 93 (NUP93) and revealed its importance in influenza virus replication. NUP93 knockdown mediated by siRNAs reduced viral replication and decreased the efficiency of the early step of the viral life cycle. NUP93 did not appear to be important for virus binding, internalization, or the nuclear import of viral ribonucleoprotein (vRNP); however, in NUP93-depleted cells, viral RNA accumulated in the nucleus. These results suggest that NUP93 is involved in the nuclear export of viral RNA.
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页数：9
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