EFFECT OF FIBROBLAST CELL LINES ON CIPROFLOXACIN LOADED COMPOSITE SCAFFOLD FOR BONE REGENERATION

Osteomyelitis is a bone infection and inflammation caused by bacteria. Osteomyelitis often requires surgery and prolonged antibiotic for weeks or months The aim of this proposed work is provide a cost-effective biomaterial that can be implanted at the target site to effectively eradicate the disease. One of the downside of this disease is the recurrence of the disease, even after treatment. The ciprofloxacin loaded hydroxyapatite/agarose scaffold is synthesized and characterized to selectively target the diseased area, to prevent the recurrence of the disease by providing local sustained antibiotic drug delivery which is considered advantageous over the conventional methods. The biomaterial is prepared in a way to make it biocompatible by using the materials like Hydroxyapatite (HAp), which mimics the natural bone composition; Agarose, which is a natural polymer that provides excellent mechanical strength and porous structure to help cell ingrowth and Ciprofloxacin, which is an anti-bacterial drug that helps to treat the disease. The prepared material is characterized using Fourier Transform Infra-Red spectroscopy (FT-IR) and X-Ray Diffraction (XRD). The biocompatibility test is done by MTT Assay to determine the morphology changes, cell viability and cell membrane integrity.