Rapid Fluorescence Spectroscopic Characterization of Salivary DNA of Normal Subjects and OSCC Patients Using Ethidium Bromide

被引：6

作者：

Yuvaraj, Manoharan ^{[1
]}

Aruna, Prakasarao ^{[1
]}

Koteeswaran, Dornadula ^{[2
]}

Tamilkumar, Palraj ^{[3
]}

Ganesan, Singaravelu ^{[1
]}

机构：

[1] Anna Univ, Dept Med Phys, Madras 600025, Tamil Nadu, India

[2] Meenakshi Ammal Dent Coll & Hosp, Dept Oral Med & Radiol, Chennai 600095, Tamil Nadu, India

[3] Dr Rai Mem Canc Inst, Chennai 600018, Tamil Nadu, India

来源：

JOURNAL OF FLUORESCENCE | 2015年 / 25卷 / 01期

关键词：

Saliva mass screening; Salivary DNA; Ethidium bromide; Fluorescence steady and excited state kinetics; Simple and rapid technique; CANCER; BINDING; BLOOD;

D O I：

10.1007/s10895-014-1482-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently, deoxyribonucleic acid (DNA) based biomarker(s) detection has been employed for cancer diagnosis. Earlier reports have suggested the presence of more DNA in the saliva of oral squamous cell carcinoma (OSCC) than normal by electrophoresis technique. Based on these, steady state and excited state kinetics of salivary DNA has been performed with 27 normal subjects and 67 OSCC patients saliva using ethidium bromide as a probe to look for the possibility in discrimination between them. On statistical analysis the sensitivity and specificity of 88.9 and 94.0 % has been achieved from the fluorescence emission spectra and 88.9 and 92.5 % with that of fluorescence excitation.

引用

页码：79 / 85

页数：7

共 33 条

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