Fusarium solani: A New Pathogen Causing Postharvest Lemon Rot in Changchun, China

被引:2
|
作者
Fu, Y. P. [1 ]
Liu, Z. H. [2 ]
Li, Y. [1 ]
机构
[1] Jilin Agr Univ, Chinese Minist Educ Edible & Med Fungi, Engn Res Ctr, Changchun 130118, Peoples R China
[2] Shenyang Agr Univ, Dept Pathol, Coll Plant Protect, Shenyang 110866, Peoples R China
关键词
1ST REPORT;
D O I
10.1094/PDIS-08-16-1194-PDN
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Lemon (Citrus limon) is an excellent fruit renowned for its high citric acid and vitamin C, and widely used in food and cosmetic industries. Postharvest fruit loss is a major factor leading to China’s rapid decline in lemon production, after recording unprecedented high yields from 2007 to 2009. During 2015 and 2016, we collected rotting lemons from Chenxing market (43.48°N, 125.24°E), Changchun, Jilin Province. The incidence was about 5 to 10%. Symptoms initially appeared as light- to dark-brown blemishes with small amounts of white mycelium on the lemons’ blossom ends. Lesions gradually expanded to encircle the blossom ends. Finally, the whole fruit rotted; white mycelium covered the entire fruit, emitting a fermenting-like odor. We isolated pathogens from 30 samples. A single spore from each sample was cultured on potato dextrose agar (PDA) and incubated at 25°C. Colonies appeared oyster-white with loose, fluffy mycelium. To produce conidia, the pathogen was cultured on carnation leaf agar (CLA) (Liu et al. 2016) under a 12/12-h dark/light photoperiod at 25°C. Microconidia were abundant and oval, with 0 to 1 septum and dimensions ranging from 6.1 to 11.7 × 3.1 to 5.5 μm (average = 8.9 × 3.9 μm). Macroconidia were sickle-shaped, with 1 to 3 septa and typical foot-shaped basal cells; dimensions ranged from 18.1 to 45.5 × 3.6 to 7.3 μm (average = 33.9 × 5.4 μm). Chlamydospores were circular- to ovate-shaped, formed singly or in pairs, and exhibited both smooth and unsmooth textures. Based on morphological characteristics (Nelson et al. 1983), the pathogen was identified as Fusarium solani. We performed PCR amplifications of the internal transcribed spacer (ITS) region of rDNA and the translation elongation factor 1-alpha (TEF-1α) genes, using genomic DNA of representative isolate CL12 with primer pairs ITS4/ITS5 and EF1/EF2. Results of ITS sequencing (562 bp, KX497027) demonstrated 99% positive identification of F. solani (AM412637). The TEF-1α sequencing (719 bp, KX497028) also demonstrated 99% positive identification of F. solani (KF255497). Thus, based on morphological and molecular characteristics, we confirmed the pathogen as F. solani. Three independent pathogenicity tests were performed. For each test, we surface sterilized 10 fresh, nonblemished lemons and then inserted sterile needles to make 1-mm deep wounds. Lemons were then surface disinfected before inoculation with a spore suspension concentrated at 106conidia/ml of CL12 isolate (grown at 25°C for 7 days on PDA). Each inoculation site was covered with wet cotton wool. Lemons were wrapped in plastic bags and stored in a growth chamber at 25°C, 90% relative humidity, and a daily 12-h photoperiod. After 5 days, inoculated lemons displayed symptoms similar to the lemons observed in our initial study. Pathogens were consistently reisolated from infected lemons. Control lemons displayed no symptoms. An Argentina study reported postharvest lemon rot caused by F. oxysporum (Fogliata et al. 2013). To our knowledge, we are the first to report F. solani-caused postharvest lemon rot in China. An effective monitoring program and appropriate postharvest handling and storage methods are needed to prevent further economic losses. © 2017, American Phytopathological Society. All rights reserved.
引用
收藏
页码:1548 / 1549
页数:2
相关论文
共 50 条
  • [31] Emergence of Fusarium solani Causing Root Rot of Pistachio Trees in Tunisia
    Triki, M. A.
    Rhouma, A.
    Chaabouni, A. C.
    Ioos, R.
    V INTERNATIONAL SYMPOSIUM ON PISTACHIOS AND ALMONDS, 2011, 912 : 717 - 721
  • [32] First Report of Fusarium solani Causing Fruit Rot of Strawberry in Pakistan
    Mehmood, N.
    Riaz, A.
    Jabeen, N.
    Anwaar, S.
    Hussain, S. Z.
    Abbas, M. F.
    Zhang, X.
    Rosli, H.
    Gleason, M. L.
    PLANT DISEASE, 2017, 101 (09) : 1681 - 1681
  • [33] First Report of Fusarium solani Causing Stem and Root Rot on Foeniculum vulgare in Jilin Province of China
    Liu, X. C.
    Lu, B. H.
    Wang, X.
    Gao, J.
    PLANT DISEASE, 2016, 100 (01) : 210 - 210
  • [34] Characterization, phylogeny and pathogenicity of Fusarium solani causing quinoa basal stem rot in Shanxi Province, China
    Yin, Hui
    Tian, Miao
    Peng, Yu-Fei
    Chen, Ya-Lei
    Qin, Nan
    Lue, Hong
    Sun, Jiang-Wei
    Ren, Lu
    Zhao, Xiao-Jun
    PLANT PATHOLOGY, 2024, 73 (02) : 272 - 291
  • [35] First Report of Fusarium solani Species Complex Causing Root Rot of Loquat (Eriobotrya japonica) in China
    Wu, D.
    Zhang, D. H.
    Wang, C. X.
    Wei, Y.
    Timko, M. P.
    Liang, G. L.
    PLANT DISEASE, 2021, 105 (05) : 1562 - 1562
  • [36] A novel pathogen Fusarium cuneirostrum causing common bean (Phaseolus vulgaris) root rot in China
    Deng, Dong
    Wu, Wenqi
    Duan, Canxing
    Sun, Suli
    Zhu, Zhendong
    JOURNAL OF INTEGRATIVE AGRICULTURE, 2024, 23 (01) : 166 - 176
  • [37] A novel pathogen Fusarium cuneirostrum causing common bean(Phaseolus vulgaris) root rot in China
    Dong Deng
    Wenqi Wu
    Canxing Duan
    Suli Sun
    Zhendong Zhu
    Journal of Integrative Agriculture, 2024, 23 (01) : 166 - 176
  • [38] First Report of Fusarium solani Causing Fusarium Root Rot and Stem Canker on Storage Roots of Sweet Potato in China.
    Wang, R. -Y
    Gao, B.
    Li, X. -H.
    Ma, J.
    Chen, S. -L.
    PLANT DISEASE, 2014, 98 (01) : 160 - 161
  • [39] The Isolation and Identification of a New Pathogen Causing Sunflower Disk Rot in China
    Yang, Jianfeng
    Wang, Yujie
    Shi, Shenghua
    Li, Haoyu
    Zhang, Wenbing
    Addrah, Mandela Elorm
    Zhang, Jian
    Zhao, Jun
    AGRONOMY-BASEL, 2024, 14 (07):
  • [40] First Report of Fusarium fujikuroi Causing Postharvest Rot of Kiwifruit (Actinidia chinensis) in Jiangxi Province, China
    Pan, Liuyi
    Gao, Zhu
    Mao, Jipeng
    Lin, Mengfei
    Zhang, Xiaoli
    Wang, Xiaoling
    Chen, Qinghong
    PLANT DISEASE, 2024, 108 (08)