Study on Process Optimization for Bioemulsifier Production

被引：2

作者：

Wang, Manman ^{[2
]}

Wang, Xingbiao ^{[1
]}

Zheng, Chenggang ^{[3
]}

Chang, Yunkang ^{[1
]}

Wang, Yongli ^{[4
]}

Huang, Zhiyong ^{[1
]}

机构：

[1] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin 300308, Peoples R China

[2] Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China

[3] Ptrl Explorat & Prod Res Inst, SINOPEC, Beijing 100083, Peoples R China

[4] Chinese Acad Sci, Lanzhou Inst Geol, Lanzhou 730000, Peoples R China

来源：

PROCEEDINGS OF THE 2012 INTERNATIONAL CONFERENCE ON APPLIED BIOTECHNOLOGY (ICAB 2012), VOL 1 | 2014年 / 249卷

基金：

中国国家自然科学基金;

关键词：

Bioemulsifier; Emulsification activity; Fermentation optimization; Geobacillus sp; BACILLUS-SUBTILIS; CELL-SURFACE; POTENTIAL APPLICATIONS; ARTHROBACTER RAG-1; BIOSURFACTANT; BACTERIA; RHAMNOLIPIDS; HYDROCARBONS; DEGRADATION; EMULSIFIER;

D O I：

10.1007/978-3-642-37916-1_38

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A useful bioemulsifier was secreted by Geobacillus sp. XS2 isolated from an oil-contaminated soil sample from Yumen oil field in northern China. The extracellular bioemulsifier was able to form a stable oil/water emulsion. The effects of different bioemulsifier concentrations on cell growth and activity of fermentation broth with water-soluble and water-insoluble carbon sources were studied. Various carbon sources, nitrogen sources, and C/N ratios were screened, and the culture conditions such as pH and ventilation were optimized through the fermentation progress. After a series of steps of optimization, the maximum bioemulsifier production reached to 21.1 g/L +/- 1.15 g/L and more than four times higher than that observed in the initial experiment. And the bacterial strain XS2 showed a good performance to adapt to the environment and strong potential for industrial applications.

引用

页码：367 / 379

页数：13

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