Study on Process Optimization for Bioemulsifier Production

被引：2
|
作者：
Wang, Manman [2 ]
Wang, Xingbiao [1 ]
Zheng, Chenggang [3 ]
Chang, Yunkang [1 ]
Wang, Yongli [4 ]
Huang, Zhiyong [1 ]
机构：
[1] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin 300308, Peoples R China
[2] Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China
[3] Ptrl Explorat & Prod Res Inst, SINOPEC, Beijing 100083, Peoples R China
[4] Chinese Acad Sci, Lanzhou Inst Geol, Lanzhou 730000, Peoples R China
来源：
PROCEEDINGS OF THE 2012 INTERNATIONAL CONFERENCE ON APPLIED BIOTECHNOLOGY (ICAB 2012), VOL 1 | 2014年 / 249卷
基金：
中国国家自然科学基金;
关键词：
Bioemulsifier; Emulsification activity; Fermentation optimization; Geobacillus sp; BACILLUS-SUBTILIS; CELL-SURFACE; POTENTIAL APPLICATIONS; ARTHROBACTER RAG-1; BIOSURFACTANT; BACTERIA; RHAMNOLIPIDS; HYDROCARBONS; DEGRADATION; EMULSIFIER;
D O I：
10.1007/978-3-642-37916-1_38
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
A useful bioemulsifier was secreted by Geobacillus sp. XS2 isolated from an oil-contaminated soil sample from Yumen oil field in northern China. The extracellular bioemulsifier was able to form a stable oil/water emulsion. The effects of different bioemulsifier concentrations on cell growth and activity of fermentation broth with water-soluble and water-insoluble carbon sources were studied. Various carbon sources, nitrogen sources, and C/N ratios were screened, and the culture conditions such as pH and ventilation were optimized through the fermentation progress. After a series of steps of optimization, the maximum bioemulsifier production reached to 21.1 g/L +/- 1.15 g/L and more than four times higher than that observed in the initial experiment. And the bacterial strain XS2 showed a good performance to adapt to the environment and strong potential for industrial applications.
引用
收藏
页码：367 / 379
页数：13
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