Rapid determination of 93 banned industrial dyes in beverage, fish, cookie using solid-supported liquid-liquid extraction and ultrahigh-performance liquid chromatography quadrupole orbitrap high-resolution mass spectrometry

Banned industrial dyes are composed of a large number of chemicals with diverse physical and chemical properties, making their simultaneous determination a challenging task. A one-step extraction and purification of 93 banned industrial dyes from beverage, fish and cookie sample methods was proposed by using solid supported liquid-liquid extraction (SLE). The extract was analyzed by ultrahigh-performance liquid chromatography quadrupole orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap-HRMS). The quantitative and qualitative mode adopts Q-Orbitrap-HRMS full scan MS (full scan MS1) and data-dependent MS/MS (dd-MS2) acquisition mode. The mass resolution was screened under 70,000 FWHM for full-scan MS1 and 35,000 FWHM for dd-MS2. Linearity was observed in the range of 0.01~0.5 mu g/mL and the limits of quantification were 0.04~0.2 mg/kg for 93 dyes. The average recoveries were 70.5-105.8%, with RSD <= 10%. The proposed method has the ability to simultaneously screen many banned dyes in foods with high throughput, sensitivity and reliability.