Photochemical immobilization of protein on inner wall of microchannel

被引：14
|
作者：
Nakajima, H
Ishino, S
Masuda, H
Shimosaka, T
Nakagama, T
Hobo, T
Uchiyama, K
机构：
[1] Tokyo Metropolitan Univ, Grad Sch Engn, Dept Appl Chem, Hachioji, Tokyo 1920397, Japan
[2] Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki 3058563, Japan
来源：
CHEMISTRY LETTERS | 2005年 / 34卷 / 03期
关键词：
D O I：
10.1246/cl.2005.358
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel strategy for patterning several kinds of proteins in a specific position of a microchannel is presented. Horseradish peroxidase (HRP) and alkaline phosphatase (ALP) are covalently immobilized on a polystyrene microchannel surface through a photoreactive crosslinker with an azide group at one end and a succinimide group at the other by UV irradiation. The activities of HRP and ALP are evaluated using Amplex Red and Fluorescein diphosphate, respectively. The fluorescence has been observed only at the enzyme-immobilized position.
引用
收藏
页码：358 / 359
页数：2
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