A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

被引：20

作者：

Tajika, Yuki ^{[1
]}

Murakami, Tohru ^{[1
]}

Iijima, Keiya ^{[2
]}

Gotoh, Hiroki ^{[3
]}

Takahashi-Ikezawa, Maiko ^{[4
]}

Ueno, Hitoshi ^{[1
]}

Yoshimoto, Yuhei ^{[2
]}

Yorifuji, Hiroshi ^{[1
]}

机构：

[1] Gunma Univ, Grad Sch Med, Dept Anat, 39-22 Showa Machi 3 Chome, Maebashi, Gunma 3718511, Japan

[2] Gunma Univ, Grad Sch Med, Dept Neurosurg, 39-22 Showa Machi 3 Chome, Maebashi, Gunma 3718511, Japan

[3] Nagoya Univ, Grad Sch Bioagr Sci, Lab Sericulture & Entomoresources, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648601, Japan

[4] Gunma Univ, Grad Sch Hlth Sci, Dept Occupat Therapy, 39-22 Showa Machi 3 Chome, Maebashi, Gunma 3718511, Japan

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

OPEN-SOURCE SOFTWARE; 3-DIMENSIONAL RECONSTRUCTION; BRAIN; ANATOMY;

D O I：

10.1038/s41598-017-03900-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have developed an imaging method designated as correlative light microscopy and block-face imaging (CoMBI), which contributes to improve the reliability of morphological analyses. This method can collect both the frozen sections and serial block-face images in a single specimen. The frozen section can be used for conventional light microscopic analysis to obtain 2-dimensional (2D) anatomical and molecular information, while serial block-face images can be used as 3-dimensional (3D) volume data for anatomical analysis. Thus, the sections maintain positional information in the specimen, and allows the correlation of 2D microscopic data and 3D volume data in a single specimen. The subjects can vary in size and type, and can cover most specimens encountered in biology. In addition, the required system for our method is characterized by cost-effectiveness. Here, we demonstrated the utility of CoMBI using specimens ranging in size from several millimeters to several centimeters, i.e., mouse embryos, human brainstem samples, and stag beetle larvae, and present successful correlation between the 2D light microscopic images and 3D volume data in a single specimen.

引用

页数：12

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