Structure of the O-specific polysaccharide of Proteus vulgaris O45 containing 3-acetamido-3,6-dideoxy-D-galactose

被引：7
|
作者：
Perepelov, AV [1 ]
Bartodziejska, B
Senchenkova, SN
Shashkov, AS
Rozalski, A
Knirel, YA
机构：
[1] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 119991, Russia
[2] Univ Lodz, Inst Microbiol & Immunol, Dept Bacterial Immunol, PL-90237 Lodz, Poland
来源：
CARBOHYDRATE RESEARCH | 2003年 / 338卷 / 04期
关键词：
Proteus vulgaris; lipopolysaccharide; O-antigen; bacterial polysaccharide structure; 3-acetamido-3,6-dideoxy-D-galactose;
D O I：
10.1016/S0008-6215(02)00361-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Proteus vulgaris O45 and studied by sugar and methylation analyses along with H-1 and C-13 NMR spectroscopy, including 2D COSY, TOCSY, ROESY, H-detected H-1, C-13 HSQC and HMBC experiments. The following structure of the pentasaccharide repeating unit of the polysaccharide was established: -->6)-alpha-D-GlcpNAc-(1-->4)-alpha-D-GalpNAc-(1-->4)-alpha-D-GalpA-(1-->3)-beta-D-GlcpNAc-(1-->2)-beta-D-Fucp3NAc4Ac-(1--> where Fuc3NAc4Ac is 3-acetamido-4-O-acetyl-3,6-dideoxygalactose. A cross-reactivity of anti-P. vulgaris O45 serum was observed with several other Proteus lipopolysaccharides, which contains Fuc3N derivatives. (C) 2002 Published by Elsevier Science Ltd.
引用
收藏
页码：327 / 331
页数：5
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