Screening of a specific monoclonal antibody against and detection of Listeria monocytogenes whole cells using a surface plasmon resonance biosensor

In this study, a specific monoclonal antibody against Listeria monocytogenes was screened using an SPR biosensor. Monoclonal antibodies were bound to protein L, after which the L. monocytogenes cells were subjected to an affinity assay. Protein L was immobilized on a carboxymethyl dextran (CM-Dex) surface via an amine coupling method, and utilized repeatedly by regeneration. The monoclonal antibody, 'A18', was selected and employed for the high-sensitivity detection of L. monocytogenes. Under optimized conditions, 10(3) cells/mL or 50 cells were detected by the SPR biosensor. (c) KSBB.