Effect of denervation on the content of cardiac troponin-T and cardiac troponin-I in rat skeletal muscle

被引:8
|
作者
Fredericks, Salim
Degens, Hans
McKoy, Godfrina
Bainbridge, Katie
Collinson, Paul O.
Coulton, Gary
Elmahdi, Habab
Holt, David W.
机构
[1] Univ Brunei Darussalam, Inst Med, BE-1410 Gadong, Brunei
[2] Human Movement Manchester Metropolitan Univ, Inst Biophys & Clin Res, Nijmegen, Netherlands
[3] Radboud Univ Nijmegen Med Ctr, Dept Physiol, NL-6500 HB Nijmegen, Netherlands
[4] St Georges Healthcare NHS Trust, London SW17 0QT, England
关键词
cardiac troponin-T; cardiac troponin-I; skeletal muscle; denervation;
D O I
10.1016/j.clinbiochem.2006.12.008
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Abnormal rhabdomyocyte expression of cardiac troponin-T (cTnT) was thought to interfere with the cTnT assay. cTnT isoforms have been shown to be transiently expressed in skeletal muscle during development and in response to muscle denervation. The effect of denervation and aging on cTnT and cardiac troponin-I (cTnI) content in fast and slow rat skeletal muscles was assessed quantitatively. Sections of the tibial nerve were transected from one hind limb of both young (n = 12) and old (n = 12)rats. Animals were sacrificed at 1, 2, and 4 weeks after the operation, and the extensor digitorum longus (EDL) and the soleus were removed from both the denervated and the contralateral control limb. There was no significant difference in cTnI content between the fast EDL and slow soleus muscles. The cTnT content was significantly higher in the soleus than the EDL muscle (p < 0.001). These data, combined with data on other models in the literature, indicate that re-expression of cTnT and cTnI isoforms in adult skeletal muscle is unlikely and does not interfere with cTnT assays for assessment of cardiac damage. (c) 2007 Published by The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码:423 / 426
页数:4
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