Unraveling the Composition of the Root-Associated Bacterial Microbiota of Phragmites australis and Typha latifolia

被引:47
|
作者
Pietrangelo, Laura [1 ,2 ]
Bucci, Antonio [1 ]
Maiuro, Lucia [3 ]
Bulgarelli, Davide [2 ]
Naclerio, Gino [1 ]
机构
[1] Univ Molise, Dept Biosci & Terr, Campobasso, Italy
[2] Univ Dundee, Sch Life Sci, Plant Sci, Dundee, Scotland
[3] Univ Molise, Dept Agr Environm & Food Sci, Campobasso, Italy
来源
关键词
rhizoplane; bacteria; microbiota; biofilm; Phragmites; Typha; phytodepuration; wetlands; WATER-QUALITY; PLANTS; DIVERSITY; BIOFILM;
D O I
10.3389/fmicb.2018.01650
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Phragmites australis and Typha latifolia are two macrophytes commonly present in natural and artificial wetlands. Roots of these plants engage in interactions with a broad range of microorganisms, collectively referred to as the microbiota. The microbiota contributes to the natural process of phytodepuration, whereby pollutants are removed from contaminated water bodies through plants. The outermost layer of the root corpus, the rhizoplane, is a hot-spot for these interactions where microorganisms establish specialized aggregates designated biofilm. Earlier studies suggest that biofilm-forming members of the microbiota play a crucial role in the process of phytodepuration. However, the composition and recruitment cue of the Phragmites, and Typha microbiota remain poorly understood. We therefore decided to investigate the composition and functional capacities of the bacterial microbiota thriving at the P. australis and T. latifolia root-soil interface. By using 16S rRNA gene Illumina MiSeq sequencing approach we demonstrated that, despite a different composition of the initial basin inoculum, the microbiota associated with the rhizosphere and rhizoplane of P. australis and T. latifolia tends to converge toward a common taxonomic composition dominated by members of the phyla Actinobacteria, Firmicutes, Proteobacteria, and Planctomycetes. This indicates the existence of a selecting process acting at the root-soil interface of these aquatic plants reminiscent of the one observed for land plants. The magnitude of this selection process is maximum at the level of the rhizoplane, where we identified different bacteria enriched in and discriminating between rhizoplane and rhizosphere fractions in a species-dependent and -independent way. This led us to hypothesize that the structural diversification of the rhizoplane community underpins specific metabolic capabilities of the microbiota. We tested this hypothesis by complementing the sequencing survey with a biochemical approach and scanning electron microscopy demonstrating that rhizoplane-enriched bacteria have a bias for biofilm-forming members. Together, our data will be critical to facilitate the rational exploitation of plant-microbiota interactions for phytodepuration.
引用
收藏
页数:13
相关论文
共 50 条
  • [21] Effect of Wastewater on the Composition of Bacterial Microbiota of Phragmites australis Used in Constructed Wetlands for Phytodepuration
    Cangioli, Lisa
    Salobehaj, Maria
    Del Duca, Sara
    Fagorzi, Camilla
    Berardi, Chiara
    Coppini, Ester
    Fibbi, Donatella
    Fani, Renato
    Vassallo, Alberto
    PLANTS-BASEL, 2022, 11 (23):
  • [22] Synchrotron micro-scale measurement of metal distributions in Phragmites australis and Typha latifolia root tissue from an urban brownfield site
    Feng, Huan
    Qian, Yu
    Gallagher, Frank J.
    Zhang, Weiguo
    Yu, Lizhong
    Liu, Changjun
    Jones, Keith W.
    Tappero, Ryan
    JOURNAL OF ENVIRONMENTAL SCIENCES, 2016, 41 : 172 - 182
  • [23] Synchrotron micro-scale measurement of metal distributions in Phragmites australis and Typha latifolia root tissue from an urban brownfield site
    Huan Feng
    Yu Qian
    Frank J.Gallagher
    Weiguo Zhang
    Lizhong Yu
    Changjun Liu
    Keith W.Jones
    Ryan Tappero
    Journal of Environmental Sciences, 2016, (03) : 172 - 182
  • [24] INTERACTIONS BETWEEN PHRAGMITES-AUSTRALIS (CAV) TRIN EX STEUD AND TYPHA-LATIFOLIA L
    SZCZEPANSKA, W
    SZCZEPANSKI, A
    EKOLOGIA POLSKA-POLISH JOURNAL OF ECOLOGY, 1982, 30 (1-2): : 165 - 186
  • [25] Treatment of industrial wastewater with two-stage constructed wetlands planted with Typha latifolia and Phragmites australis
    Calheiros, Cristina S. C.
    Rangel, Antonio O. S. S.
    Castro, Paula M. L.
    BIORESOURCE TECHNOLOGY, 2009, 100 (13) : 3205 - 3213
  • [26] Morphological adaptations of emergent plants to water flow:: a case study with Typha angustifolia, Zizania latifolia and Phragmites australis
    Asaeda, T
    Fujino, T
    Manatunge, J
    FRESHWATER BIOLOGY, 2005, 50 (12) : 1991 - 2001
  • [27] Nutrient removal potential and biomass production by Phragmites australis and Typha latifolia on European rewetted peat and mineral soils
    Geurts, Jeroen J. M.
    Oehmke, Claudia
    Lambertini, Carla
    Eller, Franziska
    Sorrell, Brian K.
    Mandiola, Samuel R.
    Grootjans, Albert P.
    Brix, Hans
    Wichtmann, Wendelin
    Lamers, Leon P. M.
    Fritz, Christian
    SCIENCE OF THE TOTAL ENVIRONMENT, 2020, 747
  • [28] Canopy structure, photosynthetic capacity and nitrogen distribution in adjacent mixed and monospecific stands of Phragmites australis and Typha latifolia
    J. N. Hirtreiter
    D. L. Potts
    Plant Ecology, 2012, 213 : 821 - 829
  • [29] Canopy structure, photosynthetic capacity and nitrogen distribution in adjacent mixed and monospecific stands of Phragmites australis and Typha latifolia
    Hirtreiter, J. N.
    Potts, D. L.
    PLANT ECOLOGY, 2012, 213 (05) : 821 - 829
  • [30] Different Denitrification Capacity in Phragmites australis and Typha latifolia Sediments: Does the Availability of Surface Area for Biofilm Colonization Matter?
    Soana, Elisa
    Vincenzi, Fabio
    Gavioli, Anna
    Castaldelli, Giuseppe
    WATER, 2025, 17 (04)