In vitro stimulation of human influenza-specific CD8+ T cells by dendritic cells pulsed with an influenza virus-like particle (VLP) vaccine

被引：36

作者：

Song, Haifeng ^{[1
]}

Wittman, Vaughan ^{[2
]}

Byers, Anthony ^{[2
]}

Tapia, Tenekua ^{[2
]}

Zhou, Bin ^{[1
]}

Warren, William ^{[2
]}

Heaton, Penny ^{[1
]}

Connolly, Kevin ^{[1
]}

机构：

[1] Novavax Inc, Rockville, MD 20850 USA

[2] VaxDesign Inc, Orlando, FL 32826 USA

来源：

VACCINE | 2010年 / 28卷 / 34期

关键词：

CD8+T cells; Dendritic cells; Virus-like particle (VLP) vaccine; PROTECTIVE IMMUNE-RESPONSES; NECROSIS-FACTOR-ALPHA; CROSS-PRESENTATION; ANTIBODY-RESPONSE; LEISHMANIA-MAJOR; A VIRUS; GAMMA; NEURAMINIDASE; ACTIVATION; EFFICACY;

D O I：

10.1016/j.vaccine.2010.06.044

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The purpose of this in vitro study was to determine if a virus-like particle (VLP) influenza vaccine stimulated human CM T cells in a dendritic cell (DC): T cell co-culture system. VLP-pulsed DCs were co-cultured with autologous CD8(+) T cells from five donors. Functional CD8(+) T cells were detected via cell surface and intracellular cytokine staining. T cells from four of the five donors showed >= 2-fold increase over background in the % activated CD8(+). cells. These results indicate that the influenza VLP vaccine can stimulate CD8(+) T cells via DC antigen presentation, likely through the MHC-I pathway, thus broadening the immunological response induced by this promising influenza vaccine. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：5524 / 5532

页数：9

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