Studies on characteristics and adsorption of BSA on hydrogel biomaterials

Hydrogel biomaterials were synthesized by radical copolymerization of N-vinyl pyrrolidone (NVP) and 2-hydroxyethylmathacrylate (HEMA), with azobisisoutyronitrile (AIBN) as an initiator, reacting at 60 similar to 70 degrees C for 24 hours, which were designed as contact lens due to the good chemical stability and high biocompatibility. The absorbency of bovine serum albumin (BSA) was measured by the ultraviolet spectrophotometer. The influence of pH, initial protein concentration and ionic strength were investigated in detail. The results showed that the absorption of protein on hydrogel biomaterials increased with the immersing time increasing, and was stable during 4 days. The absorption of protein on hydrogel increased with the equilibrium water content increasing. The protein absorption on hydrogels reduced the permeability of the oxygen of the biomaterials.